The Gene Expression Center provides global gene expression (transcriptome) analysis and genome-wide association studies (GWAS) services using cutting edge next generation sequencing (Illumina HiSeq2000 and Roche 454 FLX) and microarray (Affymetrix, and Illumina ) technologies. A variety of instrumentation is available for independent access, and training is available. The UW Gene Expression Center is located at 425 Henry Mall, Room 3250 in the UW Biotechnology Center.
We are happy to provide consultation for interested researchers.
spite of the growth of Next Gen Sequencing, Microarrays are still a
faster and cheaper
options in many cases, including gene expression analysis. Microarrays
are also "high throughput" and can be a challenge to biologists who need
to incorporate techniques from statistics, bioinformatics and computer
science into their routine tool kits.
course has an emphasis on "hands-on" but fundamentals will be reviewed
in lecture format, including essential statistics necessary for
microarrays. The course is geared towards molecular biologists and
mathematical or computation scientists entering
the field of bioinformatics.
will run specific "hands on" worked examples and build self-sufficient
competence using an open source software (R/Bioconductor) and one or two
commercial packages (ArrayStar, Partek Genomic Suite) as "proof of
concepts" to understand methods and algorithms presented in class.
qPCR services are available!
See our Roche Light Cycler Products and Services page for more information.
Illumina iScan System services are available!
Please see our iScan services page for details.
Next Generation sequencing: The Gene Expression Center in
collaboration with the DNA Sequencing Facility is proud to offer next generation sequencing services. Next
generation sequencing applications for Small RNA , directional RNA sequencing, and RNA are available on Illumina's HiSeq 2000.
Illumina iScan system: The Gene Expression Center now offers services for Illumina’s iScan system. The iScan system supports a wide variety BeadChips for researchers needing high throughput options for high-density genotyping, CNV analysis, DNA methylation, and gene expression profiling.
Affymetrix GeneChips: Affymetrix GeneChip technology provides efficient access to genetic information using high-density oligonucleotide probe arrays. Affymetrix manufactures their arrays by employing technologies adapted from the semiconductor industry, photolithography and solid-phase chemistry. These arrays contain hundreds of thousands of unique oligonucleotide probes that are designed to maximize sensitivity, specificity, and reproducibility. An Affymetrix GeneChip Analysis System is available for a variety of functional genomic studies performed with any Affymetrix GeneChip. The Center offers two options for using this system: Full GeneChip Labeling & Hybridization Service and Hybridization and Scanning Service .
The Gene Expression Center's Advisory Committee Members are Audrey Gasch (Laboratory of Genetics), Michael Newton (Department of Bioinformatics and Medical Informatics) Jeff Johnson (School of Pharmacy) and Alan Attie (Department of Biochemistry).
Biotechnology Bldg at 425 Henry Mall, Room 3250 Madison, WI 53706
ph:(608)-265-3029 fax:(608)-262-6748 firstname.lastname@example.org