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The
factors that are most important for the maintenance
of the pluripotency of embryonic stem (ES) cells
include the transcription factors OCT4 and DPPA4.
I propose to study the global DNA recognition
properties of these essential transcription
factors on a genome-wide scale. I will map OCT4
and DPPA4 binding by performing ChIP-chip experiments
which use chromatin immunopreciptated (ChIP)
DNA using an antibody of interest to probe a
microarray slide (chip) containing genomic DNA
to identify sites of protein occupancy in cells.
I intend to apply detailed knowledge of cis-regulatory
elements to dissect gene regulatory networks
that control stem cell fate and manipulate critical
nodes externally with engineered regulators.
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