Invitrogen
Supply Centers
University
of Wisconsin
Biotechnology
Center
2-221 and Babcock
Hall 215
January 2008 |

|
General
Instructions: You
must have a valid external purchase order number to purchase products.
Biotechnology
Center
Supply
Center
Hours: Open,
as long as you can get into the Biotechnology
Center
.
Babcock
Supply
Center
Instructions and Hours: The
key may be obtained in room 223. Supply Center hours are 9:00
am to 5:00 pm
Monday through Friday. Questions, call Ilenys Diaz at 263-2004.
Other
questions and suggestions for inventory: Please
contact your local Invitrogen Account Manager,
Cindy Komro by voice mail at (800) 955-6288, extension 61441.
For FBS questions, contact George Strebel at extension 46939.
Modifying
Enzymes and PCR Products
| BioTech
|
Product
Description |
Cat.
No. |
Size
|
Price
|
| -20° C |
Bovine
Serum Albumin |
15561-020
|
150
mg |
158.11 |
| -20° C |
DNase
I, Amplification Grade - For superior RT-PCR |
18068-015
|
100
u |
82.30
|
| -20° C |
DNase
I - Non-detectable levels of RNase activity |
18047-019
|
20,000
u |
256.68 |
| -20° C |
DNA
Polymerase I |
18010-017
|
250
u |
77.92 |
| -20° C |
E.
coli
DNA Ligase |
18052-019
|
100
u |
35.08 |
| -20° C |
Elongase®
Enzyme Mix - Offers longest PCR, higher yields and high fidelity
|
10480-010
|
100
rxns |
127.19 |
| -20° C |
Klenow
(DNA Polymerase I, Large Fragment) |
18012-021
|
100
u |
82.95 |
| -20° C |
M-MLV
Reverse Transcriptase |
28025-013
|
40,000
u |
178.11 |
| -20° C |
PCR
SuperMix - Ready-to-use mixture |
10572014
|
100
rxns |
79.62 |
| -20° C |
Platinum®
PCR SuperMix - Ready-to-use mixture |
11306-016
|
100
rxns |
109.65 |
| -20° C |
Platinum®
Pfx DNA Polymerase- Highest fidelity, automatic
hot start |
11708-013
|
100
rxns |
97.35 |
| -20° C |
Platinum®
Pfx DNA Polymerase |
11708-021
|
250
rxns |
228.80 |
| -20° C |
Platinum®
Quantitative PCR SuperMix-UDG |
11730-017
|
100
rxns |
161.19 |
| -20° C |
Platinum®
SYBR® Green qPCR SuperMix-UDG |
11733-038
|
100
rxns |
177.30 |
| -20° C |
Platinum®
Taq DNA Polymerase - Automatic hot start |
10966-018
|
100
rxns |
69.42 |
| -20° C |
Platinum®
Taq DNA Polymerase - Automatic hot start |
10966-034
|
500
rxns |
303.20 |
| -20° C |
Platinum®
Taq High Fidelity - Enzyme blend offers high fidelity,
high yields and automatic hot start |
11304-011
|
100
rxns |
118.27 |
| -20° C |
Proteinase K - RNase, DNase Free |
25530-015 |
100 mg |
60.38
|
| -20° C |
Proteinase K, Solution (20mg/ml) |
25530-015 |
5 mL |
103.19 |
| -20° C |
Ribonuclease
H |
18021-014
|
30
u |
91.98 |
-20° C |
Ribonuclease H |
18021-071 |
120 u |
346.92 |
| -20° C |
RNaseOUTT
Recombinant RNase Inhibitor |
10777-019
|
5,000
u |
94.08 |
-20° C |
Second-Strand Buffer |
10812-014 |
0.5 mL |
86.24 |
| -20° C |
SuperScriptT
II RNase H - Reverse Transcriptase |
18064-014
|
10,000
u |
201.60 |
| -20° C |
SuperScript™
III RNase H - Reverse Transcriptase |
18080-044
|
10,000
u |
199.92 |
| -20° C |
SuperScript™
Double-Stranded cDNA Synthesis Kit |
11917-010
|
10
rxns |
767.27 |
| -20° C |
SuperScript™
First Strand Synthesis System for RT-PCR |
11904-018
|
50
rxns |
305.04 |
| -20° C |
SuperScript™
III First Strand Synthesis System for RT-PCR |
18080-051
|
50
rxns |
302.68 |
| -20° C |
SuperScript™
III One-Step RT-PCR w/ Platinum Taq
|
12574-018
|
25
rxns |
140.62 |
| -20° C |
T4
DNA Ligase (1 unit/microl) |
15224-017
|
100
u |
43.71 |
| -20° C |
T4
DNA Ligase, high concentration (5 units/microl) |
15224-041
|
250
u |
85.69 |
| -20° C |
T4
DNA Polymerase |
18005-017
|
50
u |
51.43 |
-20° C |
T4 DNA Polymerase |
18005-025 |
250 u |
206.78 |
| -20° C |
T4
Kinase |
18004-010
|
200
u |
107.89 |
| -20° C |
T7
RNA Polymerase |
18033-019
|
2,500
u |
139.04 |
| -20° C |
Taq
DNA Polymerase, Recombinant - PCR Licensed |
10342-053
|
100
u |
42.00 |
| -20° C |
Taq
DNA Polymerase, Recombinant - PCR Licensed |
10342-020
|
500
u |
148.77 |
Restriction
Endonucleases
BioTech
|
Product
|
Cat.
No. |
Size
|
Price
|
-20° C |
Bgl
II |
15213-010
|
400
u |
26.52 |
-20° C |
Eco
R
I |
15202-013
|
5,000
u |
22.44 |
-20° C |
Hin
d
III |
15207-012
|
5,000
u |
18.36 |
-20° C |
Nhe
I |
15444-011
|
300
u |
53.95 |
-20° C |
Not
I |
15441-025
|
200
u |
48.91 |
-20° C |
Sph I |
15413-016 |
200 u |
83.40 |
-20° C |
Xba I |
15226-012 |
2,000 u |
41.40 |
-20° C |
Xho I |
15231-012 |
2,500 u |
25.46 |
Molecular
Weight Markers, Nucleotides, and Nucleic Acids
BioTech
|
Product
Description |
Cat.
No. |
Size
|
Price
|
-20° C |
10
mM dNTP Mix - each nucleotide at 10 mM conc. |
18427-013
|
100
ul |
58.65 |
-20° C |
100
mM dNTP Set - 25 umol each of dATP, dCTP, dGTP, dTTP |
10297-018
|
4 x 25 umol |
189.84
|
-20° C |
0.5-10kb
RNA Ladder |
15623-200 |
75
ug |
114.92 |
-20° C |
1
Kb DNA Ladder |
15615-016
|
250
ug |
118.24 |
-20° C |
Trackit™ 1 Kb DNA Ladder |
10488-072 |
100
app |
78.20 |
-20° C |
1
Kb Plus DNA Ladder (20 bands from 100 bp to 12 kb)
|
10787-018
|
250
ug |
117.28 |
-20° C |
100
bp DNA Ladder (100 bp to 1,500 bp) |
15628-019
|
50
ug |
80.91 |
|
Trackit™ 100 bp DNA Ladder |
10488-058 |
100 app |
84.80 |
-20° C |
BenchMark™
Prestained Protein Ladder (10 to 220 kDa) |
10748-010
|
2
x 250 ul |
107.88 |
-20° C |
BenchMark™
Protein Ladder (10 to 220 kDa) |
10747-012
|
2
x 250 m l |
91.14 |
-20° C |
Low
Mass DNA Ladder (100 bp to 1,500 bp) |
10068-013
|
200
ul
|
89.18 |
-20° C |
MagicMark™
XP Western Standard (20-220 kDa) |
LC5602
|
250
ul |
216.69 |
-20° C |
Novex Sharp Pre-Stained Protein Standard |
LC5800 |
2 x 250ul |
121.52 |
-20° C |
Oligo
(dT)12-18 Primer |
18418-012
|
25
ug |
82.56 |
-20° C |
Oligo
(dT)20 Primer (Optimal with SuperScript® III RT) |
18418-020
|
15
ug |
107.18 |
-20° C |
Random
Primers |
48190-011
|
9
A260 U |
116.19 |
4° C |
SeeBlue®
Pre-Stained Protein Standard |
LC5625
|
500
ul |
119.56 |
4° C |
SeeBlue®
Plus2 Pre-Stained Protein Standard |
LC5925
|
500
ul |
120.28 |
|
Supercoiled
DNA Ladder |
15622-012
|
25
ug |
118.80 |
-20° C |
Yeast tRNA |
15401-011 |
25 mg |
84.06 |
UltraPure™ Reagents
BioTech
|
Product
Description |
Cat.
No. |
Size
|
Price
|
RT
|
UltraPure™ Agarose |
15510-019
|
100
g |
109.12 |
RT
|
UltraPure™ Agarose |
15510-027
|
500
g |
373.15 |
4° C |
UltraPure™ Buffer-saturated Phenol |
15513-047
|
400
ml |
186.66 |
-20° C |
IPTG |
15529-019 |
100
ml |
36.48 |
4° C |
TRIzol®
Reagent |
15596-026
|
100
ml |
110.70 |
4° C |
TRIzol®
Reagent |
15596-018
|
200
ml |
211.20 |
-20° C |
X-gal
|
15520-034
|
100
mg |
52.28 |
Transfection
Reagents - visit
www.invitrogen.com for
lipid selection
BioTech
|
Product
Description |
Cat.
No. |
Size
|
Price |
4° C
|
Lipofectamine™ 2000 Transfection Reagent |
11668-027 |
0.75 mL |
218.24 |
4° C |
Lipofectamine™ 2000 Transfection Reagent |
11668-019 |
1.5 mL |
418.00 |
4° C |
Lipofectamine™ LTX Reagent |
15338-100 |
1.0 mL |
385.02 |
4° C |
Lipofectamine™ Transfection Reagent |
18324-012 |
1.0 mL |
214.54 |
4° C |
Oligofectamine™ Reagent |
12252-011 |
1.0 mL |
209.49 |
4° C |
Opti-MEM® I Reduced-Serum Medium (1X) liquid |
31985-070 |
500 mL |
24.20 |
4° C |
PLUS™ Reagent |
11514-015 |
0.85 mL |
134.58 |
RT
|
S.O.C.
Medium |
15544-034
|
10
x 10 ml |
60.00 |
NuPAGE
Protein Electrophoresis Precast Gels and Supplies
BioTech
|
Product
Description |
Cat.
No. |
Size
|
Price |
RT
|
NuPAGE®
10% Bis-Tris Gel, format: 1 mm, 10 well |
NP0301BOX
|
Box
of 10 gels |
99.05 |
RT
|
NuPAGE®
10% Bis-Tris Gel, format: 1 mm, 15 well |
NP0303BOX
|
Box
of 10 gels |
99.05 |
RT
|
NuPAGE®
4-12% Bis-Tris Gel, format: 1 mm, 10 well |
NP0321BOX
|
Box
of 10 gels |
99.05 |
RT
|
NuPAGE®
4-12% Bis-Tris Gel, format: 1 mm, 15 well |
NP0323BOX
|
Box
of 10 gels |
99.05 |
RT |
NuPAGE® 4-12% Bis-Tris Gel, format: 1.5 mm, 15 well |
NP0336BOX |
Box of 10 gels |
99.05 |
RT |
NuPAGE® Novex 12% Bis-Tris Gel, format: 1 mm, 15 well |
NP0343BOX |
Box of 10 gels |
99.05 |
4° C |
NuPAGE®
MOPS SDS Running Buffer (20X) |
NP0001
|
500
ml |
50.40 |
4° C |
NuPAGE®
MES SDS Running Buffer (20X) |
NP0002
|
500
ml |
50.40 |
4° C |
NuPAGE®
Antioxidant |
NP0005
|
15
ml |
18.12 |
4° C |
NuPAGE®
Transfer Buffer (20X) |
NP0006 |
125
ml |
17.10 |
4° C |
NuPAGE®
LDS Sample Buffer (4X) |
NP0007
|
10
ml |
9.37 |
RT
|
SimplyBlue™
SafeStain |
LC6060
|
1
L |
97.65 |
Molecular Probes Secondary Antibody Conjugates and Gel Stains, Visit--www.invitrogen.com/antibodies
BioTech
|
Product
Description |
Cat.
No. |
Size
|
Price |
4° C |
Alexa Fluor® 488 goat anti-rabbit IgG (H+L) *2 mg/mL* |
11593-019
|
20
ml |
129.36 |
RT
|
SimplyBlue™ Safetaint |
LC6060 |
1 L |
97.65 |
| 4° C |
Streptavidin, R-phycoerythrin conjugate (SAPE) *1 mg/mL* |
S866 |
1 mL |
223.44 |
| -20 ° C |
SYBR® Green I nucleic acid gel stain (10,000X) |
S7563 |
500 ul |
193.26 |
| RT |
SYPRO® Ruby protein gel stain, 1 L |
S1200 |
1 L |
95.40 |
| RT |
SYBR® Safe DNA gel stain in DMSO (10,000X) |
S33102 |
400 ul |
45.00 |
GIBCO™ Cell Culture Media, Balanced Salt Solutions and Fetal Bovine Serum
BioTech
|
Product
Description |
Cat.
No. |
Size
|
Price |
4° C |
D-MEM
(1X), High Glucose, w/ 4.5 g/ L, D-glucose, L-glut, w/o Na
pyruvate |
11965-092
|
500
ml |
1 6.34
10+ 4.88 |
4° C |
D-MEM
(1X), Low Glucose, w/ 1.0 g/ L D-glucose, L-glut, w/ Na pyruvate
|
11885-084
|
500
ml |
6.99 |
RT |
D-PBS
(1X), w/o calcium & magnesium |
14190-144
|
500
ml |
1 5.63
10+ 4.56 |
4° C |
F12
Nutrient Mixture (1X) w/ L-glutamine |
11765-054
|
500
ml |
10.07
|
-20° C |
Fetal
Bovine Serum, Qualified ( Effective through 6-30-04
) |
26140-079
|
500
ml |
220.73 |
4° C |
MEM
(1X), w/ Earle's salts and L-glutamine |
11095-080
|
500
ml |
5.69 |
4° C |
Opti -MEM®
I Reduced Serum Media |
31985-070
|
500
ml |
24.20 |
4° C |
RPMI
Medium 1640 (1X), w/ L-glutamine |
11875-093
|
500
ml |
1 6.62
10+ 4.88
|
GIBCO™ Cell Culture Reagents and Antibiotics
BioTech
|
Product
Description |
Cat.
No. |
Size
|
Price |
-20° C |
Antibiotic-Antimycotic
(100X) |
15240-062
|
100
ml |
22.63
|
-20° C |
Fungizone®
Antimycotic, liquid |
15290-018
|
20
ml |
16.72 |
RT
|
Geneticin®
(G418), powder |
11811-031
|
5
g |
226.98
|
-20° C |
Geneticin®
(G418) liquid (50 mg/ml) |
10131-035
|
20
ml |
87.13 |
-20° C |
L-Glutamine-200
mM (100X) |
25030-081
|
100
ml |
1-3
12.60
4+
11.00 |
4° C |
Hygromycin
B , liquid |
10687-010
|
20
ml |
144.35 |
-20° C |
Penicillin-Streptomycin
(100X) - 10,000 U of pen. G and 10,000 mg streptomycin sulfate/ml
|
15140-122
|
100
ml |
1-3
11.97
4+
10.17 |
| -20° C |
Recovery™ Cell Culture Freezing Medium (replaces Cell Culture Freezing Medium, cat. 11101-011) |
12648-010 |
50 ml |
82.35 |
-20° C |
Trypsin-EDTA
(0.05% Trypsin, 0.53 mM EDTA) (1X) |
25300-054
|
100
ml |
1-4
6.99
5+
6.20 |
-20° C |
Trypsin-EDTA
(0.25% Trypsin, 1 mM EDTA) (1X) |
25200-056
|
100
ml |
1-4
6.99
5+
6.20 |
-20° C |
ZeocinT
|
R250-01
|
1
g |
186.79 |
Prices
are subject to change.
For
more information,
please
contact your local Invitrogen Account Manager,
Cindy
Komro, at (800) 955-6288, extension 61441
or
FBS specialist George Strebel at extension 46939.
|