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Invitrogen Supply Centers

University of Wisconsin

Biotechnology Center 2-221 and Babcock Hall 215

January 2008

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General Instructions:   You must have a valid external purchase order number to purchase products.  

Biotechnology Center Supply Center Hours:   Open, as long as you can get into the Biotechnology Center .  

Babcock Supply Center Instructions and Hours:   The key may be obtained in room 223. Supply Center hours are 9:00 am to 5:00 pm Monday through Friday.   Questions, call Ilenys Diaz at 263-2004.

Other questions and suggestions for inventory:   Please contact your local Invitrogen Account Manager,
Cindy Komro by voice mail at (800) 955-6288, extension 61441.   For FBS questions, contact George Strebel at extension 46939.
 

 

Modifying Enzymes and PCR Products

BioTech

Product Description

Cat. No.

Size

Price

-20° C

Bovine Serum Albumin

15561-020

150 mg

158.11

-20° C

DNase I, Amplification Grade   - For superior RT-PCR

18068-015

100 u

82.30

-20° C

DNase I - Non-detectable levels of RNase activity

18047-019

20,000 u

256.68

-20° C

DNA Polymerase I

18010-017

250 u

77.92

-20° C

E. coli DNA Ligase

18052-019

100 u

35.08

-20° C

Elongase® Enzyme Mix - Offers longest PCR, higher yields and high fidelity

10480-010

100 rxns

127.19

-20° C

Klenow (DNA Polymerase I, Large Fragment)   

18012-021

100 u

82.95

-20° C

M-MLV Reverse Transcriptase

28025-013

40,000 u

178.11

-20° C

PCR SuperMix - Ready-to-use mixture

10572014

100 rxns

79.62

-20° C

Platinum® PCR SuperMix - Ready-to-use mixture

11306-016

100 rxns

109.65

-20° C

Platinum® Pfx DNA Polymerase- Highest fidelity, automatic hot start

11708-013

100 rxns

97.35

-20° C

Platinum® Pfx DNA Polymerase

11708-021

250 rxns

228.80

-20° C

Platinum® Quantitative PCR SuperMix-UDG

11730-017

100 rxns

161.19

-20° C

Platinum® SYBR® Green qPCR SuperMix-UDG

11733-038

100 rxns

177.30

-20° C

Platinum® Taq DNA Polymerase - Automatic hot start

10966-018

100 rxns

69.42

-20° C

Platinum® Taq DNA Polymerase - Automatic hot start

10966-034

500 rxns

303.20

-20° C

Platinum® Taq High Fidelity - Enzyme blend offers high fidelity, high yields and automatic hot start

11304-011

100 rxns

118.27

  -20° C

Proteinase K - RNase, DNase Free

25530-015

100 mg

60.38

  -20° C Proteinase K, Solution (20mg/ml)   25530-015        5 mL          103.19

-20° C

Ribonuclease H

18021-014

30 u

91.98

-20° C

Ribonuclease H

18021-071

120 u

346.92

-20° C

RNaseOUTT Recombinant RNase Inhibitor

10777-019

5,000 u

94.08

-20° C

Second-Strand Buffer

10812-014

0.5 mL

86.24

-20° C

SuperScriptT II RNase H - Reverse Transcriptase

18064-014

10,000 u

 201.60

-20° C

SuperScript™ III RNase H - Reverse Transcriptase

18080-044

10,000 u

199.92

-20° C

SuperScript™ Double-Stranded cDNA Synthesis Kit

11917-010

10 rxns

767.27

-20° C

SuperScript™ First Strand Synthesis System for RT-PCR

11904-018

50 rxns

305.04

-20° C

SuperScript™ III First Strand Synthesis System for RT-PCR

18080-051

50 rxns

302.68

-20° C

SuperScript™ III One-Step RT-PCR w/ Platinum Taq

12574-018

25 rxns

140.62

-20° C

T4 DNA Ligase (1 unit/microl)

15224-017

100 u

43.71

-20° C

T4 DNA Ligase, high concentration (5 units/microl)

15224-041

250 u

85.69

-20° C

T4 DNA Polymerase

18005-017

50 u

51.43

-20° C

T4 DNA Polymerase

18005-025

250 u

206.78

-20° C

T4 Kinase

18004-010

200 u

107.89

-20° C

T7 RNA Polymerase

18033-019

2,500 u

139.04

-20° C

Taq DNA Polymerase, Recombinant - PCR Licensed

10342-053

100 u

42.00

-20° C

Taq DNA Polymerase, Recombinant - PCR Licensed

10342-020

500 u

148.77

 

Restriction Endonucleases

BioTech

Product

Cat. No.

Size

Price

-20° C

Bgl II

15213-010

400 u

26.52

-20° C

Eco R I

15202-013

5,000 u

22.44

-20° C

Hin d III

15207-012

5,000 u

18.36

-20° C

Nhe I

15444-011

300 u

53.95

-20° C

Not I

15441-025

200 u

48.91

-20° C

Sph I

15413-016

200 u

83.40

-20° C

Xba I

15226-012

2,000 u

41.40

-20° C

Xho I

15231-012

2,500 u

25.46

 


Molecular Weight Markers, Nucleotides, and Nucleic Acids

BioTech

Product Description

Cat. No.

Size

Price

-20° C

10 mM dNTP Mix - each nucleotide at 10 mM conc.

18427-013

100 ul

58.65

-20° C

100 mM dNTP Set - 25 umol each of dATP, dCTP, dGTP, dTTP

10297-018

  4 x 25 umol

189.84

-20° C

0.5-10kb RNA Ladder

15623-200

75 ug

114.92

-20° C

1 Kb DNA Ladder

15615-016

250 ug

118.24

-20° C

Trackit 1 Kb DNA Ladder

10488-072

100 app

78.20

-20° C

1 Kb Plus DNA Ladder   (20 bands from 100 bp to 12 kb)

10787-018

250 ug

117.28

-20° C

100 bp DNA Ladder   (100 bp to 1,500 bp)

15628-019

50 ug

80.91

 

Trackit 100 bp DNA Ladder

10488-058

100 app

84.80

-20° C

BenchMark Prestained Protein Ladder   (10 to 220 kDa)

10748-010

2 x 250 ul

107.88

-20° C

BenchMark Protein Ladder   (10 to 220 kDa)

10747-012

2 x 250 m l

91.14

-20° C

Low Mass DNA Ladder   (100 bp to 1,500 bp)

10068-013

200 ul

89.18

-20° C

MagicMark XP Western Standard (20-220 kDa)

LC5602

250 ul

216.69

-20° C

Novex Sharp Pre-Stained Protein Standard

LC5800

2 x 250ul

121.52

-20° C

Oligo (dT)12-18 Primer

18418-012

25 ug

82.56

-20° C

Oligo (dT)20 Primer (Optimal with SuperScript® III RT)

18418-020

15 ug

107.18

-20° C

Random Primers

48190-011

9 A260 U

116.19

4° C

SeeBlue® Pre-Stained Protein Standard

LC5625

500 ul

119.56

4° C

SeeBlue® Plus2 Pre-Stained Protein Standard

LC5925

500 ul

120.28

 

Supercoiled DNA Ladder

15622-012

25 ug

118.80

-20° C

Yeast tRNA

15401-011

25 mg

84.06

 

UltraPure Reagents

BioTech

Product Description

Cat. No.

Size

Price

RT

UltraPure Agarose

15510-019

100 g

109.12

RT

UltraPure Agarose

15510-027

500 g

   373.15

4° C

UltraPure Buffer-saturated Phenol

15513-047

400 ml

186.66

-20° C

IPTG

15529-019

100 ml

36.48

4° C

TRIzol® Reagent

15596-026

100 ml

110.70

4° C

TRIzol® Reagent

15596-018

200 ml

211.20

-20° C

X-gal

15520-034

100 mg

52.28

         

 

Transfection Reagents - visit www.invitrogen.com for lipid selection

BioTech

Product Description

Cat. No.

Size

  Price

   4° C 

Lipofectamine 2000 Transfection Reagent

11668-027

0.75 mL

218.24

4° C

Lipofectamine 2000 Transfection Reagent

11668-019

1.5 mL

418.00

 4° C

Lipofectamine LTX Reagent

15338-100

1.0 mL

385.02

4° C

Lipofectamine Transfection Reagent

18324-012

1.0 mL

214.54

4° C

OligofectamineReagent

12252-011

1.0 mL

209.49

 4° C

Opti-MEM® I Reduced-Serum Medium (1X) liquid

31985-070

500 mL

24.20

 4° C

PLUSReagent

11514-015

0.85 mL

134.58

RT

S.O.C. Medium

15544-034

10 x 10 ml

60.00

 

NuPAGE Protein Electrophoresis Precast Gels and Supplies

BioTech

Product Description

Cat. No.

Size

  Price

RT

NuPAGE® 10% Bis-Tris Gel, format:   1 mm, 10 well

NP0301BOX

Box of 10 gels

99.05

RT

NuPAGE® 10% Bis-Tris Gel, format:   1 mm, 15 well

NP0303BOX

Box of 10 gels

99.05

RT

NuPAGE® 4-12% Bis-Tris Gel, format:   1 mm, 10 well

NP0321BOX

Box of 10 gels

99.05

RT

NuPAGE® 4-12% Bis-Tris Gel, format:   1 mm, 15 well

NP0323BOX

Box of 10 gels

99.05

RT

NuPAGE® 4-12% Bis-Tris Gel, format:   1.5 mm, 15 well

NP0336BOX

Box of 10 gels

99.05

RT

NuPAGE® Novex 12% Bis-Tris Gel, format:   1 mm, 15 well

NP0343BOX

Box of 10 gels

99.05

4° C

NuPAGE® MOPS SDS Running Buffer (20X)

NP0001

500 ml

50.40

4° C

NuPAGE® MES SDS Running Buffer (20X)

NP0002

500 ml

50.40

4° C

NuPAGE® Antioxidant

NP0005

15 ml

18.12

4° C

NuPAGE® Transfer Buffer (20X)

      NP0006

125 ml

17.10

4° C

NuPAGE® LDS Sample Buffer (4X)

NP0007

10 ml

9.37

RT

SimplyBlue SafeStain

LC6060

1 L

97.65

 

Molecular Probes Secondary Antibody Conjugates and Gel Stains, Visit--www.invitrogen.com/antibodies

BioTech

Product Description

Cat. No.

Size

  Price

4° C

Alexa Fluor® 488 goat anti-rabbit IgG (H+L) *2 mg/mL*

11593-019

20 ml

129.36

RT

SimplyBlue Safetaint

LC6060

1 L

97.65

    4° C Streptavidin, R-phycoerythrin conjugate (SAPE) *1 mg/mL*        S866          1 mL               223.44
  -20 ° C SYBR® Green I nucleic acid gel stain (10,000X)        S7563        500 ul               193.26
     RT SYPRO® Ruby protein gel stain, 1 L        S1200            1 L                 95.40
     RT SYBR® Safe DNA gel stain in DMSO (10,000X)      S33102        400 ul                 45.00

 

GIBCO Cell Culture Media, Balanced Salt Solutions and Fetal Bovine Serum

BioTech

Product Description

Cat. No.

Size

  Price

4° C

D-MEM (1X), High Glucose, w/ 4.5 g/ L, D-glucose, L-glut, w/o Na pyruvate

11965-092

500 ml

  1     6.34

  10+   4.88

4° C

D-MEM (1X), Low Glucose, w/ 1.0 g/ L D-glucose, L-glut, w/ Na pyruvate

11885-084

500 ml

       6.99

RT

D-PBS (1X), w/o calcium & magnesium

14190-144

500 ml

     1      5.63

   10+    4.56

4° C

F12 Nutrient Mixture (1X) w/ L-glutamine

11765-054

500 ml

10.07

-20° C

Fetal Bovine Serum, Qualified    ( Effective through 6-30-04 )

26140-079

500 ml

220.73

4° C

MEM (1X), w/ Earle's salts and L-glutamine

11095-080

500 ml

      5.69

4° C

Opti -MEM® I Reduced Serum Media

31985-070

500 ml

24.20

4° C

RPMI Medium 1640 (1X), w/ L-glutamine

11875-093

500 ml

   1        6.62

    10+   4.88  

 

GIBCO Cell Culture Reagents and Antibiotics

BioTech

Product Description

Cat. No.

Size

  Price

-20° C

Antibiotic-Antimycotic (100X)

15240-062

100 ml

22.63

-20° C

Fungizone® Antimycotic, liquid

15290-018

20 ml

16.72

RT

Geneticin® (G418), powder

11811-031

5 g

226.98

-20° C

Geneticin® (G418) liquid (50 mg/ml)

10131-035

20 ml

87.13

-20° C

L-Glutamine-200 mM (100X)

25030-081

100 ml

1-3     12.60

4+      11.00

4° C

Hygromycin B , liquid  

10687-010

20 ml

144.35

-20° C

Penicillin-Streptomycin (100X) - 10,000 U of pen. G and 10,000 mg streptomycin sulfate/ml

15140-122

100 ml

1-3     11.97

4+      10.17

  -20° C Recovery Cell Culture Freezing Medium (replaces Cell Culture Freezing Medium, cat. 11101-011) 12648-010        50 ml          82.35

-20° C

Trypsin-EDTA (0.05% Trypsin, 0.53 mM EDTA) (1X)

25300-054

100 ml

1-4       6.99

5+      6.20

-20° C

Trypsin-EDTA (0.25% Trypsin, 1 mM EDTA) (1X)

25200-056

100 ml

1-4      6.99

5+      6.20

-20° C

ZeocinT

R250-01

1 g

186.79

Prices are subject to change.

 

For more information,

please contact your local Invitrogen Account Manager,

Cindy Komro, at (800) 955-6288, extension 61441

or FBS specialist George Strebel at extension 46939.