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Mass Spectrometry / Proteomics Facility:
The University of Wisconsin Biotechnology Center has acquired several mass spectrometers for the analysis of biomolecules, including proteins, peptides, oligonucleotides, oligosaccharides and other small organic and inorganic molecules. In addition to measuring the molecular weight of compounds, the instruments are designed to perform MS/MS so that the structure of a selected ion can be deduced from the fragments. This is particularly useful for peptide sequencing and post-translational modification mapping. The ESI-MS are equipped with HPLC systems which are used with reversed phase (RP) nanobore / capillary columns, for LC/MS/MS experiments.We also have an LC-MALDI spotter for combining separations with MALDI-TOF/TOF analysis. LC/MS/MS is extremely useful for quantitation of known compounds and for the rapid screening of a mixture to identify the components of interest. We also perform proteolytic digestions of proteins, which can be further analyzed by HPLC, MS, LC/MS and MS/MS for peptide mapping and peptide sequencing.
Mass Spec Class Chem 638 Lab1 Lab2
Instrumentation Overview
- Thermo Fisher Scientific LTQ Orbitrap XL
- Agilent 6460 Triple Quad (GLBRC instrument)
- Leco GCxGC-TOF MS (GLBRC instrument)
- Applied Biosystems 4800 MALDI TOF/TOF
- Applied Biosystems 3200 Q Trap
- Agilent LC/MSD TOF
- Agilent Nanoflow Proteomics Solution
- Micromass Q-TOF2
- Retired Bruker Biflex III
- Retired Sciex API 365
- Bio-Rad 2D-Electrophoresis
- Bio-Rad MultiImager
- Bio-Rad ProteomeWorks Plus
- Bio-Rad Trans-Blot SD Semi-Dry transfer Cell
Operational Overview
| Mass Spectrometry basics | MALDI Imaging | |
| In Gel Digest service | Untargeted Metabolomics | LC/MALDI |
| 2D-Gel Electrophoresis | Targeted Metabolomics | ITRAQ |
| 2D-LC MS/MS service | UWCCC Poster | Data Server |
Sample Submission form (pdf file)
In Gel Digest Submission form (pdf file)
| Links to protein tools | Mass Spec on Campus | Other Links |
|---|---|---|
| In Gel Digest Protocol | Chemistry Mass Spec Facility | Madison Mass Spec Discussion Group |
| MALDI-MSI | School of Pharmacy | GLBRC |
| Matrix Science Mascot | Biochemistry BIF | Madison Metabolomics Consortium |
| Protein Prospector | Human Proteomics Program | |
| NCBI OMSSA | Medicago Group | |
| The GPM | Lingjun Li | ASMS |
| Delta Mass | Lloyd Smith | VMSL |
| ExPASy | Joshua Coon | ABRF |
| Scaffold Viewer | Michael Sussman | XCMS |
Contact |
Amy Harms 2262 Biotechnology Center 425 Henry Mall Madison, WI 53706 Phone: 608/262-5637 FAX: 608/262-6748 |
| E-Mail: harms@biotech.wisc.edu |
Equipment in the facility was purchased with funds from the University of Wisconsin, the National Institutes of Health (P50 GM64598, R33 DK070297),and the National Science Foundation (DBI-0520825, DBI-9977525)