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Custom Synthesis and Characterization of Peptides
The University of Wisconsin Biotechnology Center offers custom peptide synthesis and characterization of peptides. Automated syntheses are performed using an Applied Biosystems Synergy 432A, a Protein Technologies Prelude or a Protein Technologies Symphony instrument. Standard syntheses are carried out at 25-2000 µmole scale with the choice of either free carboxyl or amide at the C-terminus. Synthetic peptides are furnished in cleaved and deprotected form as lyophilized powders. Analytical HPLC characterization and mass spectrometry are supplied as part of the basic service. Purification by HPLC is also available at either a semi-preparative scale (10 mg column load) or at preparative scale (up to 100 mg column load). Synthesis of modified peptides is available, including insertion of non-standard amino acids, biotin or fluorescence labeling, cyclic peptides and incorporation of stable isotopes.
New! Online Order Form
| Contact Information |
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| E-Mail: glcase@wisc.edu |
| Basic synthesis, cleavage & deprotection Includes analytical HPLC and mass spectrometry |
Starting at $30 per residue (Difficult peptides are priced on an individual basis) Users outside the UW System add 48.5% to prices |
| Conjugation to KLH, OVA or BSA | $60 per 4 mg scale |
| Semi-Preparative HPLC purification (10 mg load) | $95 |
| Preparative scale HPLC purification (up to 100 mg load), mandatory purification for peptides 25 residues or longer | $195 |