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Hardware

High performance Cluster: for high throughput data analysis. It is not directly available to the end users, but can be used through the BRC staff.

AGAR1, Agar2, Agar3 workstations: These three servers are available, free of charge, for data analysis. They each have 20 GB of RAM and six CPUs. They have a wide variety of genomics software installed, including CLC Bio Genomics Workbench. The full list of installed softwares can be viewed in the section below. Access to these machines requires you to log in from an on-site computer, which is provided in room 1226 of the Biotechnology Center. You must contact the BRC to set up an account before you can use the computers and there is a Google calendar to sign up for time on each machine.

SUMO workstation: This is our large memory machine. It has 512 GB of RAM and 32 processors. There is a charge for using this machine, which is a sliding scale starting at $3.50 per hour, reduced to $2 per hour after a certain number of hours has been reached (pricing details upon request). The charge model is similar to a rental car; when you sign up for this machine you have exclusive use. You are charged from your first login to your last logout - these are calendar hours, not CPU hours. Remote access is allowed to this machine. There is a waiting list, please contact the BRC if you would like to be placed on the list.

High performance storage: An Isilon storage server with 400TB space for sequencing data access and storage.

Software

Analysis Suite:

Application Version  Description  Link  
CLC Genomics workbench   6.0.2  A user-friendly commercial analysis package for analyzing, comparing and visulaizing next generation sequencing data. Installed on Agar1, 2, and 3  CLC bio  
Galaxy Sever    An open source software package provides user-friendly web interface to large collection of open source tools  Galaxy Project  
454     Analysis Software for 454 sequences. Include GS de novo Assembler, GS Mapper  454  
CASAVA  1.8.2  Illumina CASAVA pipeline  Illumina  

Assembling

 Application  Version   Description  Link 
 cap3  10/15/07  DNA sequence assembly program   paper
 Ray  1.6.1  de novo assembler using MPI 2.2  Ray
 SSPACE  2.0  A stand-alone program for scaffolding pre-assembled contigs using pair-end data  Base Clear
 staden  2.0.0b9  A set of DNA sequence assembly (Gap4 and Gap5), editing and analysis tools (Spin)  Staden
 TMAP  3.0  torrent mapping alignment program  TMAP
 velvet  1.2.07  Sequence assembler for short reads  EMBL-EBI

 Alignment

Application  Version  Description  Link 
 bowtie  0.12.8  Fast, memory-efficient short read aligner  bowtie
 bowtie2  2.1.0  Fast, memory-efficient gapped aligner  bowtie2
 BWA  0.7.2-r351  Software package for mapping low-divergent sequences against a large reference genome. Consists of BWA-backtrack, BWA-SW and BWA-MEM.  BWA
 MAQ  0.6.6  Mapping and assembly with quality to reference sequences  MAQ
 SSAHA2  2.5.5  Pairwise sequence alignment program for aligning sanger, 454, illumina reads to reference genomes  Sanger
 stampy  1.0.21  Fast short reads mapper for illumina reads  Oxford
 YASRA  2.32  Performs comparative assembly of short reads using reference genome that can have substantial differences.  GACRC
       

 RNAseq

Application  Version  Description  Link 
 Cufflinks  2.0.2  assemblestranscripts, estimates their abundances, and tests for differential expression and regulation in RNA-Seq samples  
 MapSplice      
 R/EBseq      
 RSEM  1.2.4  RNA-seq by expectation-Maximization  
 STAR    Fast splice aligner   
 Tophat  2.0.8  a fast splice junction mapper for RNA-Seq reads  
 trinity    de novo transcriptome assembler  
     

Variant analysis

Application  Version  Description  Link
 annovar    variant annotation tools   
 breakdancer    structural variant detection tools  
 GATK    General purpose variant detection tools  
 snpEff      

ChIPseq:

Application  Version  Description  Link 
 homer      
 QuEST