Hardware
High performance Cluster: for high throughput data analysis. It is not directly available to the end users, but can be used through the BRC staff.
AGAR1, Agar2, Agar3 workstations: These three servers are available, free of charge, for data analysis. They each have 20 GB of RAM and six CPUs. They have a wide variety of genomics software installed, including CLC Bio Genomics Workbench. The full list of installed softwares can be viewed in the section below. Access to these machines requires you to log in from an on-site computer, which is provided in room 1226 of the Biotechnology Center. You must contact the BRC to set up an account before you can use the computers and there is a Google calendar to sign up for time on each machine.
SUMO workstation: This is our large memory machine. It has 512 GB of RAM and 32 processors. There is a charge for using this machine, which is a sliding scale starting at $3.50 per hour, reduced to $2 per hour after a certain number of hours has been reached (pricing details upon request). The charge model is similar to a rental car; when you sign up for this machine you have exclusive use. You are charged from your first login to your last logout - these are calendar hours, not CPU hours. Remote access is allowed to this machine. There is a waiting list, please contact the BRC if you would like to be placed on the list.
High performance storage: An Isilon storage server with 400TB space for sequencing data access and storage.
Software
Analysis Suite:
| Application | Version | Description | Link | |
| CLC Genomics workbench | 6.0.2 | A user-friendly commercial analysis package for analyzing, comparing and visulaizing next generation sequencing data. Installed on Agar1, 2, and 3 | CLC bio | |
| Galaxy Sever | | An open source software package provides user-friendly web interface to large collection of open source tools | Galaxy Project | |
| 454 | | Analysis Software for 454 sequences. Include GS de novo Assembler, GS Mapper | 454 | |
| CASAVA | 1.8.2 | Illumina CASAVA pipeline | Illumina | |
Assembling
| Application | Version | Description | Link |
| cap3 | 10/15/07 | DNA sequence assembly program | paper |
| Ray | 1.6.1 | de novo assembler using MPI 2.2 | Ray |
| SSPACE | 2.0 | A stand-alone program for scaffolding pre-assembled contigs using pair-end data | Base Clear |
| staden | 2.0.0b9 | A set of DNA sequence assembly (Gap4 and Gap5), editing and analysis tools (Spin) | Staden |
| TMAP | 3.0 | torrent mapping alignment program | TMAP |
| velvet | 1.2.07 | Sequence assembler for short reads | EMBL-EBI |
Alignment
| Application | Version | Description | Link |
| bowtie | 0.12.8 | Fast, memory-efficient short read aligner | bowtie |
| bowtie2 | 2.1.0 | Fast, memory-efficient gapped aligner | bowtie2 |
| BWA | 0.7.2-r351 | Software package for mapping low-divergent sequences against a large reference genome. Consists of BWA-backtrack, BWA-SW and BWA-MEM. | BWA |
| MAQ | 0.6.6 | Mapping and assembly with quality to reference sequences | MAQ |
| SSAHA2 | 2.5.5 | Pairwise sequence alignment program for aligning sanger, 454, illumina reads to reference genomes | Sanger |
| stampy | 1.0.21 | Fast short reads mapper for illumina reads | Oxford |
| YASRA | 2.32 | Performs comparative assembly of short reads using reference genome that can have substantial differences. | GACRC |
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RNAseq
| Application | Version | Description | Link |
| Cufflinks | 2.0.2 | assemblestranscripts, estimates their abundances, and tests for differential expression and regulation in RNA-Seq samples | |
| MapSplice | | | |
| R/EBseq | | | |
| RSEM | 1.2.4 | RNA-seq by expectation-Maximization | |
| STAR | | Fast splice aligner | |
| Tophat | 2.0.8 | a fast splice junction mapper for RNA-Seq reads | |
| trinity | | de novo transcriptome assembler | |
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Variant analysis
| Application | Version | Description | Link |
| annovar | | variant annotation tools | |
| breakdancer | | structural variant detection tools | |
| GATK | | General purpose variant detection tools | |
| snpEff | | | |
ChIPseq:
| Application | Version | Description | Link |
| homer | | | |
| QuEST | | | |