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DNA Sequencing Facility

DNA Sequencing Facility: DNA Extraction

The DNA Sequencing Facility provides DNA extraction services for whole blood, saliva, buccal swabs, plant tissue and mammal tissue. The UWBC DNA Sequencing Facility can also work with researchers to extract DNA from tissues that may not be listed here. Please inquire about pricing for DNA sources not listed here.

All data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequence Facility are intended for research purposes only. They are not intended or certified for diagnostic or clinical use.


Contact
Information

John Alliet

1262 Biotechnology Center

425 Henry Mall Madison WI 53706

Phone:608-262-4657 

E-Mail: jpalliet@wisc.edu

Pricing

Note: non-UW users are charged a 50.5% surcharge above rates listed below.

Input Setup fee
Per Sample fee
Example Pricing

Buccal Swabs

Very fresh, bristle-style swabs generally yield the best results. Swabs must be clearly labeled.

$190.00

Applied per set of up to 96 swabs

$3.75

$550.00  (~$5.73 per extraction)

96 buccal swabs.

Mammal Tissue

Up to 20-mg tissue in 96-well plates.

$140.00

Applied to a single 96-well plate

$4.25

$548.00 (~$5.71 per extraction)

One 96-well plate of tissue.

Oragene Saliva Kits

Contents of clearly labeled Oragene collection vessel.

$325.00

Applied per set of up to 192 collection vessles

$2.75

$853.00 (~$4.44 per extraction)

192 Oragene collection vessels.

Plant Tissue

1-cm leaf cutting, fresh or lyophilized, in 96-well plates.

$180.00

Applied to a single 96-well plate.

$1.50

$324.00 (~$3.38 per extraction)

One 96-well plate of tissue.

Whole Blood (Low-throughput)

Up to 10-mL whole blood in clearly labeled vials.

$130.00

Applied per set of up to16 vials.

$16.00

$386.00 (~$24.13 per extraction)

16 vials, whole blood (up to 10-mL).

Whole Blood (High-throughput)

 200-uL whole blood in clearly labeled vials.

$700.00

Applied per set of up to 192 vials.

$2.15

$1,112.80 (~$5.80 per extraction)

192 vials, whole blood (up to 200-uL).

Sample Submission

Please contact jpalliet@wisc.edu for sample submission instructions and scheduling information. All projects must be discussed before samples are submitted.

Policy Statement

Every effort is made to provide our users with the best data in a timely fashion for the advancement of their research. Costs and turnaround times for DNA extraction projects are dependent on the the specific type of tissue and the number of samples involved. These can be estimated for projects involving tissues for which we have already optimized DNA extraction protocols.  For all other tissues, we will be happy to evaluate and optimize the DNA extraction protocols for projects of five hundred or more samples.