Custom Oligonucleotide
Pricing and Scales
Below is a table showing pricing
and other information for the types of oligonucleotide synthesis scales
offered. To get specific information for a particular synthesis scale,
click on the header for that scale. You will then be linked to ordering
directions and information specific to that synthesis scale. Note that
while only the 50 nmol "unpurified" PCR/sequencing oligos undergo routine
quality control (QC) by Mass Spectrometry, this procedure can be carried
out on any oligo the customer desires (including those not synthesized
at this facility). The charge for this service is $5.00, and a hard copy
of the data is provided. Also note that the 50 nmol (<60mer) purified
synthesis scale at the Biotech Center requires an additional cartridge
purification step, so there is a $5.00 charge for purification of these
small scale oligos. In all other cases, an additional cost of $25 is assessed
for cartridge purification and a base of $60 for HPLC. If you have questions
please call the facility at 608-263-9882.
*not recommended for >50-mers
A newer higher throughput oligonucleotide
synthesizer now on site at the UW DNA synthesis facility allows us to
make smaller scale oligos at a greatly reduced price. These oligos are
not purified at all, and are designed for use primarily in PCR and sequencing
reactions. The Biotech Center uses these oligos regularly for in house
sequencing and PCR projects. Unlike the previous instrument we at the
Biotech Center will quantify ALL syntheses. Presently we are charging
$0.30/base, which includes desalting AND QC (there is a $1.00 set-up fee
here to cover the cost of the synthesis column). No modifications are available with this option, but unlike the
previous instrument mixed bases ARE available. We are having routine success
with 60-mers thus far, so the old length restrictions have been lifted. Again, mass spec is done on all
oligos at this scale, and there is no additional cost for this quality
control analysis--it is built into the pricing. No hard copy of the results
is routinely generated or provided to the user, but as with other synthesis
options it is available for a small fee. Click here on Mass Spectrometry for more information about the utility of this analysis
procedure. Ordering is carried out in a slightly different manner for
this option.
To Place an Order:
- Online: Go to the Online
Order Form. You need to specify the service option desired
only (specifically, "50 nmole desalted/PCR or Sequencing). We
now only offer one option for these orders...we will quantify ALL orders
in-house, the price is still $0.30/base with a $1.00 set-up fee.
Orders are supplied to the user lyophilized and with the nanomoles/vial
listed for the user. You can also click on this on-line Oligo
Calculator if you want to convert to another concentration description.
All orders placed for this option
will be cartridge purified as part of the cost, an additional $6.00 relative
to the 50 nmole non-purified is added. The instrument does NOT have
a built in purification, so we at the Biotech Center manually do a RP
cartridge purification on requested oligos. This is preferred for site-directed
mutagenesis work and raises signal to noise ratio in numerous molecular
biology applications. On the customer copy the total nanomoles/vial and micromoles are
provided for each oligo. We lyophilize the product after elution because
of the toxicity of ACN and the fact it will degrade the oligos over time.
Turnaround time for this option is typically 24-36 hours, if the order
is received before 3:00 P.M. Users are notified by telephone or e-mail when their
order is ready.
To Place an Order:
- Online: This is the sole method of ordering and will result in the
shortest turn around time; simply click on the Online
Order Form here or at the bottom of this page to place an on-line
oligo order, fill in the appropriate user information and then click
on "50 nmole purified" for desired scale. Once that is done,
choose the range for the total number of oligos desired, Go to the "Next"
page and enter the names and sequences needed, then be sure to Submit.
All modified oligos (for a list of some
of the available modifications and pricing information, click on Modifications) will be made on an ABI 394 which does not include a purification
step, unless requested. There is a $5 set-up fee per oligo. If a modification is desired, note this in the sequence using a
"Z" to designate the modified base, and make a second note of it in the
"Comments" section of the oligo order form. Multiple modifications
within a single oligonucleotide can be requested using W, X, or Y at the
appropriate position within the sequence. The oligonucleotides synthesized
in this option are analyzed by Mass Spectrometry. In addition, the synthesis of these oligos is analyzed at each
step by an integral trityl monitor in the synthesizer, so failures are
usually detected right away. At a cost of $5 to the user the Biotech Center
can run a Mass Spec Analysis of the DNA and provide a hard copy of the
output. For more information about this, click here on Mass Spectrometry. These oligonucleotides can be cartridge purified for
$25 (needs to be denoted in the "Comments" section) or HPLC
purified for $50 (simply click on the button in the order form.
Yield is decreased with purification. If you have any questions regarding
purification simply click on Cartridge Purification. Turnaround time for this option is approximately 2-3 days, but can vary
depending on modifier availability.
- Online: This is the sole method of ordering. Simply click on
the Online
Order Form here or at the bottom of this page to place an on-line
oligo order, fill in the appropriate user information and then click
on "40 nmole" for desired scale. Once that is done,
choose the range for the total number of oligos desired, Go to the "Next"
page and enter the names and sequences needed, then be sure to Submit.
It is important to note that once a modification (Y,Z, etc.,) has been
added the user will need to denote the modification in the "Comments"
section at the bottom of the order form. Once an oligo of length
>60-mer is entered the computer assumes that NO cartridge purification
is desired, unless of course the user expicitly requests it in the "Comments"
section.
Synthesis of oligonucleotides
at this larger scale, either plain or modified (for a list of some of
the available modifications and pricing information, click on Modifications) will be made on an ABI 394 which does not include a purification
step. There is a $5 set-up fee per oligo. If a modification is desired, note this in the sequence using a
"Z" to designate the modified base, and make a second note of it in the
comments section of the oligo order form. Multiple modifications within
a single oligonucleotide can be requested using W, X, or Y at the appropriate
position within the sequence. The oligonucleotides synthesized in this
option are analyzed by polyacrylamide gel electrophoresis (PAGE) to insure
that laddering of the DNA (indicating synthesis failures) is minimal.
In addition, the synthesis of these oligos is analyzed at each step by
an integral trityl monitor in the synthesizer, so failures are usually
detected right away. At a cost of $5 to the user, the Biotech Center can
run a Mass Spec Analysis of the DNA and provide a hard copy of the output.
For more information about this, click here on Mass Spectrometry. These oligonucleotides can be cartridge purified for $25 or HPLC
purified for a $50 base cost, but we need to know this before synthesis
is initiated so indicate it by clicking the relevant request on the order
form. Yield is decreased with purification. If you have any questions
regarding purification simply click on Cartridge Purification. Turnaround time for this option is approximately 2-3 days, but can
vary depending on modifier availability.
To Place an Order:
- Online: This is the sole method of ordering. Simply click on
the Online
Order Form here or at the bottom of this page to place an on-line
oligo order, fill in the appropriate user information and then click
on "0.2 micromole" for desired scale. Again, if any
purification is needed be sure to click the appropriate button on the
order form. Once that is done, choose the range for the total
number of oligos desired, Go to the "Next" page and enter
the names and sequences needed, then be sure to Submit. It is
important to note that once a modification (Y,Z, etc.,) has been added
the user will need to denote the modification in the "Comments"
section at the bottom of the order form.
For very large quantities of
DNA, the Biotech Center has available a 1.0 micromol scale synthesis for
the users. Large-scale syntheses are available with the same assortment
of modifications as the 40 and 200 nmole standard oligos, so when ordering
use the same forms. There is a $5 set-up fee per oligo. If a modification is desired, note this in the sequence
using a "Z" to designate the modified base, and make a second note of
it in the comments section of the oligo order form. Multiple modifications
within a single oligonucleotide can be requested using W, X, or Y at the
appropriate position within the sequence. The oligonucleotides synthesized
in this option are analyzed by polyacrylamide gel electrophoresis (PAGE)
to insure that laddering of the DNA (indicating synthesis failures) is
minimal. In addition, the synthesis of these oligos is analyzed at each
step by an integral trityl monitor in the synthesizer, so failures are
usually detected right away. At a cost of $5.00 to the user, the Biotech
Center can run a Mass Spec Analysis of the DNA and provide a hard copy
of the output. For more information about this, click here on Mass Spectrometry. These oligonucleotides can be cartridge purified for
$25.00, but we need to know this before synthesis is initiated so indicate
it in the "Comments" section of the order form. Yield is decreased with
purification. If you have any questions regarding purification simply
click on Cartridge Purification. Turnaround time
for the 1.0 micromol option is approximately 2-3 days, but can vary depending
on modifier availability.
To Place an Order:
- Online: This is the sole method of ordering. Simply click on
the Online
Order Form here or at the bottom of this page to place an on-line
oligo order, fill in the appropriate user information and then click
on "1.0 micromole" for desired scale. Again, if any
purification is needed be sure to click the appropriate button on the
order form. Once that is done, choose the range for the total
number of oligos desired, Go to the "Next" page and enter
the names and sequences needed, then be sure to Submit. It is
important to note that once a modification (Y,Z, etc.,) has been added
the user will need to denote the modification in the "Comments"
section at the bottom of the order form.