Modifications

Following is a list of some of the modifications we have placed onto an oligonculeotide:

Inosine
Spacer 18
Doping (i.e., mixed bases in particular ratios)
5' Amino C6
Biotin
Fluorescein (5', 3', dT)
5' FAM
5' HEX
5' TET
TAMRA dT
5' Thiol C6 (S-S)
3' Thiol C3 S-S
5' Cy3/Cy5
Branching Phosphoramidite (used to enhance signal on fluorescent labeled oligos, can be expanded to other uses on request)
5' Phosphorylation
Sulfurizing Reagent (Phosphorothioate)
5' Dioxygenin
Psoralen C2

Currently we do not offer synthesis of peptide-nucleic acid.

Also, check out the Glen Research web site www.glenres.com for a list of many other modifications possible. If they sell the precursor compound, we can probably make it for you.

Molecular probes, located at www.probes.com, also carries a wide variety of DNA modifications that we are able to provide for our users.

It is easiest to call or e-mail us if you have ANY questions regarding requests for modified oligos here at the Biotech Center. This extends to questions on pricing as well.

Pricing on oligonucleotide modifications is calculated in a straightforward manner but is variable-the base synthesis cost is exactly what it is normally charged for the scale requested (see table above), and the cost of the modifying reagent from the company is passed directly to the customer. The ultimate cost of the modification per oligonucleotide depends on how many modified oligonucleotides are requested, since once the modifying reagent is prepared for synthesis, it must all be used or discarded.

For example, if a user requests a 200 nmol scale synthesis of a single FAM labeled oligo, the cost is $1.25/base plus $165 for the reagent, so that single oligo has a high modifier cost of $165. However, approximately 6 200 nmol syntheses can be accomplished with that same bottle, so if 6 FAM labeled oligonucleotides are requested on a 200nmol scale, the per oligo modifier cost drops to approximately $30. If a user is not in a big hurry, they can request we hang onto the order until additional oligo requests with the same modifier come into the facility. For common modifiers such as FAM we typically receive a slow steady stream of requests so this is one way to save money. Please call if you have any questions about modified oligonucleotide pricing information.