Agilent 2100 BioAnalyzer

The Bioanalyzer utilizes 'Lab-on-a-Chip' technology to integrate sample preparation, fluid handling, separation, detection, and data analysis. Providing researchers with standardized methods and enhanced reproducibility. Each chip contains an interconnected set of gel-filled channels that allow for molecular sieving of nucleic acid and protein samples.

Quick and Easy Operation

*Simple chip preparation requires less than 20 minutes set-up time.
*Ready to use reagent kits.
*Only 1ul of sample required
*Only 30 minutes to run and analyze 11-12 samples.
*Data is displayed as a gel-image or electropherogram
*Automatically calculates fragment size, concentration, ribosomal RNA ratios,
*High detection sensitivity. More sensitive than a polyacrylamide gel stained with EtBr

  or  SYBR Gold.
*Built in quality control with custom array ladders.

Currently, Agilent has LabChip kits that can analyze RNA, DNA, and Proteins. Some examples of analysis that can be done are: quantitative analysis of PCR fragments, restriction fragment sizing, Cy5 labeled cRNA and cDNA. RNA analysis up to 6,000 bases. DNA analysis from 25-12,000bp. Analyze protein samples ranging in size from 14 to 200 kDa including cell lysates, column fractions, and purified proteins. The Gene Expression Center stocks both the Agilent RNA Nano and Pico kits, as well as the DNA 1000 kit.

THIS INSTRUMENT IS AVAILABLE TO ALL UW-MADISON RESEARCHERS

There is a one time training fee of $73.00 per hour not including the cost of the chip.

Interested users may buy their own kits or purchase single chips from the Center.

For more information visit the Agilent web site: http://www.agilent.com/chem/labonachip

Or contact: gecinfo@biotech.wisc.edu

Online scheduling is available for this instrument