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Gene Expression Center

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The Gene Expression Center provides global gene expression (transcriptome) analysis and genome-wide association studies (GWAS) services using cutting edge next generation sequencing (Illumina HiSeq2000 and Roche 454 FLX) and microarray (Affymetrix, and Illumina ) technologies. A variety of instrumentation is available for independent access,  and training is available. The UW Gene Expression Center is located at 425 Henry Mall, Room 3250 in the UW Biotechnology Center.

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We are happy to provide consultation for interested researchers.



  GEC News

The hands-on course "Computational Approaches to Analyzing Microarray Data" will be presented in conjuction with the GEC on the BTCI Campus 

July 24-28, 2013.


In spite of the growth of Next Gen Sequencing, Microarrays are still a faster and cheaper options in many cases, including gene expression analysis. Microarrays are also "high throughput" and can be a challenge to biologists who need to incorporate techniques from statistics, bioinformatics and computer science into their routine tool kits.

The course has an emphasis on "hands-on" but fundamentals will be reviewed in lecture format, including essential statistics necessary for microarrays. The course is geared towards molecular biologists and mathematical or computation scientists entering the field of bioinformatics.

Students will run specific "hands on" worked examples and build self-sufficient competence using an open source software (R/Bioconductor) and one or two commercial packages (ArrayStar, Partek Genomic Suite) as "proof of concepts" to understand methods and algorithms presented in class. 

 


 

qPCR services are  available! 

See our Roche Light Cycler Products and Services page for more information.  

   


 

 Illumina iScan System services are  available! 

  Please see our iScan services page for details.

 



 

Next Generation sequencing: The Gene Expression Center in collaboration with the DNA Sequencing Facility is proud to offer next generation sequencing services. Next generation sequencing applications for  Small RNA , directional RNA sequencing, and RNA are available on Illumina's HiSeq 2000.

 

Illumina iScan system: The Gene Expression Center now offers services for Illumina’s iScan system. The iScan system supports a wide variety BeadChips for researchers needing  high throughput options for high-density genotyping, CNV analysis, DNA methylation, and gene expression profiling. 


Affymetrix GeneChips: Affymetrix GeneChip technology provides efficient access to genetic information using high-density oligonucleotide probe arrays. Affymetrix manufactures their arrays by employing technologies adapted from the semiconductor industry, photolithography and solid-phase chemistry. These arrays contain hundreds of thousands of unique oligonucleotide probes that are designed to maximize sensitivity, specificity, and reproducibility. An Affymetrix GeneChip Analysis System is available for a variety of functional genomic studies performed with any Affymetrix GeneChip. The Center  offers two options for using this system: Full GeneChip Labeling & Hybridization Service and Hybridization and Scanning Service .

 

 

The Gene Expression Center's Advisory Committee Members are Audrey Gasch (Laboratory of Genetics), Michael Newton (Department of Bioinformatics and Medical Informatics) Jeff Johnson (School of Pharmacy) and Alan Attie (Department of Biochemistry).

 

   Biotechnology Bldg at 425 Henry Mall, Room 3250 Madison, WI 53706

ph:(608)-265-3029 fax:(608)-262-6748 gecinfo@biotech.wisc.edu