The Gene Expression Center provides global gene expression (transcriptome) analysis and genome-wide association studies (GWAS) services using cutting edge next generation sequencing (Illumina HiSeq2000 and Roche 454 FLX) and microarray (Affymetrix, Illumina and Roche NimbleGen) technologies. A variety of instrumentation is available for independent access, and training is available. The UW Gene Expression Center is located at 425 Henry Mall, Room 3250 in the UW Biotechnology Center.

We are happy to provide consultation for interested researchers.

GEC News

"Computational Approaches to Analyzing Microarray Data”

June 20-24, 2012

This "hands on" computer course is designed to raise students' self-sufficient

competence in applying fundamental statistics and bioinformatics methods to

microarray data for efficient data mining.

qPCR services are now available!

See our Roche Light Cycler Products and Services page for more information.

Illumina iScan System services are now available!

Please see our iScan services page for details.

Next Generation sequencing: The Gene Expression Center in collaboration with the DNA Sequencing Facility is proud to offer next generation sequencing services. Next generation sequencing applications for Small RNA , directional RNA sequencing, and RNA are available on Illumina's HiSeq 2000.

Illumina Iscan system: The Gene Expression Center now offers services for Illumina’s Iscan system. The Iscan system supports a wide variety BeadChips for researchers needing high throughput options for high-density genotyping, CNV analysis, DNA methylation, and gene expression profiling.

Affymetrix GeneChips: Affymetrix GeneChip technology provides efficient access to genetic information using high-density oligonucleotide probe arrays. Affymetrix manufactures their arrays by employing technologies adapted from the semiconductor industry, photolithography and solid-phase chemistry. These arrays contain hundreds of thousands of unique oligonucleotide probes that are designed to maximize sensitivity, specificity, and reproducibility. An Affymetrix GeneChip Analysis System is available for a variety of functional genomic studies performed with any Affymetrix GeneChip. The Center offers two options for using this system: Full GeneChip Labeling & Hybridization Service and Hybridization and Scanning Service .

NimbleGen Arrays: Roche NimbleGen array technology offers researchers customized high-density microarray products with unprecedented flexibility for genomics research. NimbleGen's Maskless Array Synthesis (MAS) technology combines digital light processing and rapid, high-yield photochemistry to shorten array fabrication time from months to hours. Roche NimbleGen's MAS technology offers a unique ability to make high-density arrays of long isothermal probes for advanced genomic analysis methods such as CGH, ChIP-chip, microbial resequencing, DNA methylation,and gene expression . The Gene Expression Center offers levels of service and support for Roche NimbleGen products: full array hybridization service for user prepared samples, independent instrument access, and training for all NimbleGen application methods. A NimbleGen System is available to support all NimbleGen application methods and application methods development.

The Gene Expression Center's Advisory Committee Members are Audrey Gasch (Laboratory of Genetics), Michael Newton (Department of Bioinformatics and Medical Informatics) Jeff Johnson (School of Pharmacy) and Alan Attie (Department of Biochemistry).

Biotechnology Bldg at 425 Henry Mall, Room 3250 Madison, WI 53706

ph:(608)-265-3029 fax:(608)-262-6748 gecinfo@biotech.wisc.edu