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bioanalyzer




Agilent 2100 Bioanalyzer:


The Agilent 2100 BioAnalyzer utilizes "Lab-on-a-Chip" technology to integrate sample preparation, fluid handling, separation, detection, and data analysis.  Agilent Technologies has Lab-on-a-Chip kits available that analyze RNA, DNA, and Proteins.



Some examples of analysis that can be done are:

  • Quantitative analysis of PCR fragments
  • Restriction fragment sizing
  • Cy 5 labeled cRNA and cDNA
  • RNA analysis up to 6,000 bp
  • DNA analysis from 25 - 12,000 bp
  • Analyze protein samples (cell lysate, column factions, and purified proteins) ranging in size from 14 - 200 kDa.

 Affymetrix recommends the 2100 BioAnalyzer for qualifying total (eukaryotic and prokaryotic) or messenger RNA.

Individuals must be trained satisfactorily by a Center Specialist prior to gaining access to the instrumentation.  There is a one time training fee of $73.00 per hour. The chip cost is not included in the cost of the training.  A maximum of 4 people maybe trained at one time. Please contact the GEC at gecinfo@biotech.wisc.edu to schedule training.

Lab-on-a-Chips Available in Center UW-Madison Non UW-Madison
Agilent RNA6000 NanoChip $35.00 contact GEC
Agilent RNA6000 PicoChip $35.70 contact GEC
 Agilent DNA 1000 Chip $32.45 contact GEC
Agilent High sensitivity DNA chip
   $52.30 *
contact GEC 

 

Price includes the Agilent Chip, all consumables, reagents and RNA ladder. 

* This chip may only be run by GEC personnel, and therefore incurs standard labor charges.

If you will be submitting samples for Agilent QC by GEC personal please submit a copy of the Agilent Sample submission form (form fillable) along with your samples.

If a lab is planning on running a large number of samples, it is recommended you purchase your own kit.