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Microinjection of a cell
Obtaining Repository Products

  • Introduction

The members of the International Knockout Mouse Consortium (IKMC) are working together to mutate all protein-coding genes in the mouse using a combination of gene trapping and gene targeting in C57BL/6 mouse embryonic stem (ES) cells. They have made targeted ES cells, frozen sperm, frozen embryos, and live mice commercially available. 

  • Getting Started
ES cell clones: Please contact Dr. Patricia Powers to get assistance in deciding which mutated clones to purchase. TAF recommends the ordering of three mutated ES cell clones; two of which will be injected. Mutated ES cell clones should be karyotyped. Mutated ES cell clones should be sent to: 

Dr. Patricia Powers
Genetics-Biotech Center Bldg
425 Henry Mall room 3210
Madison, WI, 53706

Frozen embryo/sperm: Please contact Kathy Krentz to discuss details. TAF recommends the ordering of two vials/straws. Frozen embryo/sperm should be sent to: 

Kathy Krentz 
Genetics-Biotech Center Bldg
425 Henry Mall room 3210
Madison, WI, 53706

If the mice have already been made, frozen embryos and/or sperm may be available. Before deciding to purchase frozen sperm/embryos from a repository, laboratories must be aware of all the costs involved. Samples will be shipped from the repository to TAF in a special liquid nitrogen tank. The empty shipping tank must be returned to the repository after delivery of samples. Domestic shipping costs ~ $200, international shipping costs ~ $500. Some repositories charge a fee/sample. Live offspring will be recovered from frozen sperm via in vitro fertilization.  
  • Repository Links