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SEE Biotech | |
| From The University of Wisconsin-Madison/Extension | ||
| Sponsored by a grant from USDA/CSREES/IFAFS | ||
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Shotgun Optical Maps of the Whole Escherichia coli O157:H7 Genome |
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Alex Lim | |||
| E. coli O157:H7: Escherichia coli O157:H7 EDL933, produces a shiga-like toxin causing over 100,000 cases of human illness annually in the United States alone and poses a significant threat to public health worldwide. Most cases are associated with strains of the serotype O157:H7 and 85% of these are linked to contaminated food. We have constructed XhoI optical map of E.coli O157 solely from genomic DNA molecules to provide a uniquely valuable scaffold for contig closure and sequence validation. Our approach obviated the need for the analysis of clones, PCR products, and hybridizations, since the maps were constructed from ensembles of single DNA molecules. | |||
| Speaker Biography | Speaker Contacts | Overall Agenda | |
| Copyright © 2003 The Board of Regents of the University of Wisconsin System. /Extension Page created November 7, 2002 Last updated November 13, 2002 Hosted by the UWBC Web server |
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