qPCR Service at the Gene Expression Center:
The UWBC Gene Expression Center offers
qPCR services utilizing the Roche LightCycler® 480 Real-Time PCR System.
Multiple levels of service are available to meet all campus researcher needs. Service options include:
- Independent access to the Roche 480 at $30 per plate (training cost of $90 per hour)
- A full service where the user provides the RNA or DNA and the Center will design and optimize the assay(s) as needed
- User provides source material and primer/probes of interest and Center will perform assays as needed and
- User submits a ready-to-go plate that is run by GEC staff on the instrument.
Roche Light Cycler 480 independent instrument usage
The Roche LightCycler 480 is available to use independently on a fee for use basis. Users must be trained by GEC staff prior to first usage. Cost of training is $90 / hour. Please contact GEC staff at firstname.lastname@example.org to schedule a training session.
|Roche LightCycler 480 || |
|User ready to go plate || |
| $45.90/plate |
Please use account# 55185396 when ordering from the Roche website to insure UW contract pricing.
GEC Custom qPCR assay design service
The GEC follows the guidelines outlined in the MIQE Guidelines when designing qPCR experiments. When RNA is being quantified, at least 2-3 normalization genes are recommended and all controls are run. Quantification/detection of gDNA is also possible with the Roche480 system and appropriate controls will be determined with the user.
Both SybrGreen and probe based assays are available based on what is appropriate for the experiment. High Melt Resolution dyes can be used with the Roche480 to distinguish size differences in the qPCR product.
Due to the difficulty with primer/probe design and testing for sensitive qPCR assays, any assay requiring primer design will be given only an approximate cost and not a firm quote. The user will be charged the actual cost of assay design and will be kept fully informed at all steps of the assay design process.
Analysis of qPCR results can be done using either the software on the Roche480 instrument or using the qBase Plus software.
All data and services provided by the University of Wisconsin Biotechnology Center Gene Expression Center are intended for research purposes only. Services are not intended nor certified for diagnostic or clinical use.
The equipment housed at the Wisconsin Biotechnology Center Gene Expression Center is intended for research purposes only. The equipment is not intended nor certified for diagnostic or clinical use.