DNA Sequencing Facility: Illumina Sequencing
DNA Sequencing Facility offers all-inclusive next generation sequencing, DNA extraction and SNP genotyping services on a rolling-submission, fee-for-service basis, with the goal of generating high-quality genomic and transcriptomic data. We work with our UW system and external collaborators to prepare DNA, RNA, ChIP, GBS, metagenomic, exome, and other sequencing libraries, provide robust sequencing data, and help interpret that data to provide meaningful biological information in a time-sensitive manner.
To discuss a project or acquire a quote, please contact our NGS team. To submit samples, please review our submission guidelines and sequencing policies, fill our our sample submission form, and contact us to set up an account and coordinate sample delivery.
New Sequencing : Partial Sequencing Lanes
New Library Preparations: Sure Select Human Exon and 16/ITS/18s/Custom Amplicon
Agilent SureSelect Human All Exon v5, 30x min coverage (6 samples min):
- Library preparation + sequencing: $800/sample (UW), $1050/sample (external)
- Library preparation, sequencing + Bioinformatics: $850/sample (UW), $1127/sample (external)
16S bacterial rRNA sequencing, v3-v4 region (48 samples min):
- Library preparation + sequencing: $50/sample (UW), $76 (external)
- Library preparation, sequencing + Bioinformatics : $65/sample, $100 (external)
Pricing listed above will be effective starting August 1, 2015.
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 53% surcharge for all labor provided; prices are as listed above. This surcharge is subject to change.
Illumina pricing is broken into two components: library preparation and run cost. Library preparation is charged on a per sample basis and varies by type. Run charges are based on the length of the sequencing run and whether it is single or paired end. Prices are subject to change due to increasing reagent costs.
Read Length: 50, 150, 250 or 300 bp; single read or paired end
Run time: 6 hrs - 3 days
Data generation per lane: 1-25 million fragments sequenced in parallel; 0.5-15 Gbases data output
Ideal for: prokaryotic ChIP-seq, smallRNA-seq, small genome resequencing and targeted capture
Illumina HiSeq 2000:
Read length: 50 or 100 bp, single read or paired end
Run time: 3-12 days
Data generation per lane: 100-200 million fragments sequenced in parallel; 7.5-35 Gbases data output
Ideal for: RNA-Seq, large genome resequencing and targeted sequencing
Illumina HiSeq 2500:
Read length: 50, 100, or 150 bp, single read or paired end
Run time: 3-12 days in standard mode, 1-5 days in rapid mode
Data generation per lane: 100-150 million fragments sequenced in parallel; 7.5-35 Gbases data output
Ideal for: de novo genome sequencing, large genome resequencing and targeted sequencing; where quick data turnaround is vital
Scheduling and Sample Submission
Please contact email@example.com for scheduling information. All projects must be discussed with a project manager prior to submitting samples. No projects will be scheduled until samples and sample submission forms have been dropped off to DNA Sequencing personnel in room 1250 Biotech Center.
All data and reagents distributed
by the University of Wisconsin Biotechnology Center DNA Sequence Facility
are intended for research purposes only. They are not intended nor
certified for diagnostic or clinical use.
Every effort is made to provide
our users with data that meets Illumina performance specifications in a timely fashion. Turnaround time for Illumina sequencing is typically 4-6 weeks. For more information, please review our Illumina sequencing policy prior to submitting samples.
Acknowledging UWBC and the DNA Sequencing Facility
A visible measure of a core's impact is made through proper acknowledgement of usage in publications. To acknowledge our core, please use the following statement: "The author(s) thank the University of Wisconsin Biotechnology Center DNA Sequencing Facility for providing ________ facilities and services”.
Support is available for projects that are run at UWBC facilities, and is provided for a fee by the UWBC Bioinformatics Resource Center. Terminals and software are available in our computer lab for clients of UWBC facilities who are internal to UW-Madison or the University of Wisconsin System who need to perform further analysis but lack the necessary hardware and software. Information on the quality reports distributed with your data is a available here.