Read accuracy and throughput make the PGM an ideal platform for resequencing or de novo
sequencing of small genomes. Improved read chemistry with the 400bp
Sequencing Kit allows for long reads lengths averaging 315-325bp
(380-400bp modal). 400bp sequencing runs done on E. coli DH10B
libraries show excellent average aligned read accuracies out past 400bp,
with longest perfect alignments well past 400bp. Data from an E. coli DH10B library prepared and sequenced in-house using the most current 400bp sequencing kit and a 314 chip can be seen here.
Read and total base throughput, coupled with rapid turnaround time, make the Proton an ideal platform for exome, transcriptome and larger genome sequencing. Chemistry improvements on the Proton have increased read throughput to 80 million reads and base throughput to 10Gb.
Torrent has made steady improvements to the read length, read quality
and analysis software for the PGM and Proton, making both platforms more viable options for many
more next-gen sequencing applications. Ion Torrent is currently
beta-testing their Ion Hi-Q Sequencing Chemistry in conjunction with
their 400bp sequencing kits. The new chemistry purportedly reduces the
indel errors that are common on this platform by 90% and will be quickly adapted on the PGM. Ion Torrent is also currently developing the PII chip for the Proton, which is stated to increase the read throughput to 200-300 million reads.