In collaboration with the DNA Sequencing Facility  the GEC offers RNA library preparation services for sequencing on the Illumina HiSeq 2500 & MiSeq.

The GEC offers library preparation services for the following assays:

TruSeq small RNA
Stranded RNA
TruSeq RNA v2
NuGEN Ovation Single Cell RNA
 (ultra-low input)
SMART-Seq v4  / Nextera XT  (ultra-low input)

Agilent Sure Select Target Enrichment

RNA Submission Guidelines 20140930


Next Generation Sequencing Submission Form


Services

Library Type Number of samples
Labor and
setup
cost
Cost per
  additional

External Rate
Labor and setup cost
External Rate cost
per Sample

TruSeq Total RNA v2   1-48 Samples* $1,450.00 $100.00 $2,065.50 $114.00
TruSeq Total RNA v2
49-96 Samples
$1,650.00 $100.00
$2371.50 $114.00


Library Type  Number of samples  Labor and
setup
cost

Cost per
Sample

External Rate
Labor and setup cost
 
External Rate cost
per Sample
 
TruSeq Stranded mRNA   1-48 Samples*
$1,235.00 $85.00
$1,759.50 $98.00
TruSeq Stranded mRNA    
49-96 Samples
$1,435.00 $85.00
$2,065.50 $98.00


 Library Type Number of samples   Labor and
setup
cost
Cost per
Sample
 
External Rate
Labor and setup cost
 
 External Rate cost
per Sample

TruSeq Stranded Total RNA 
1-48 Samples* $1,295.00 $145.00 $1,759.50
$158.00
TruSeq Stranded Total RNA  
49-96 Samples $1,495.00
$145.00
$2223.50
$158.00



  Library Type Number of samples  Labor and
setup
cost
 
Cost per
Sample
 
External Rate
Labor and setup cost
 
External Rate cost
per Sample
 
TruSeq Small RNA
1-16 Samples*
$1,540.00 $140.00
$2142.00 $160.00
TruSeq Small RNA
17-32 Samples
$380.00
$140.00
$581.40 $160.00



  Library Type

Ultra-Low RNA

Number of samples
Labor and
setup
cost

Cost per
Sample
External Rate
Labor and setup cost

External Rate cost
per Sample


Nugen Ovation SoLo
1-32 Samples*
Two PCR rounds
$1,800.00 $100.00
$2,601.00
$126.01
Clonetech SMART-Seq v4/ Nextera ST
1-96 Samples*
$1,155.00 $80.00
$1644.75
$95.30



 Other Services
Number of samples
 
Labor and
setup
cost

 Cost per
Sample
External Rate
Labor and setup cost
External Rate cost
per Sample

Ribosomal RNA Reduction 
(Ribo-Zero)

1-16 samples
$380.00 $106.00
$581.40 $109.18
RNA QC**
Per 11-12 samples
$137.00 $209.61



*Reagents included for Sample 1
** RNA is verified for quantity and quality on a NanoDrop 2000 Spectrophotometer and Agilent 2100 Bioanalyzer, respectively.  Agilent RNA chip type is dependent on concentration of RNA sample; >100ng/ul NanoChip (12 samples) & <100ng/ul PicoChip (11 samples).

Pricing listed above will be effective starting July 1, 2017. 

EXTERNAL RATES: Clients not affiliated with UW-Madison or the University of Wisconsin system are charged a 53% surcharge for all labor provided; prices are as listed above.  This surcharge is subject to change.

Illumina pricing is broken into two components: library preparation and run cost.  Library preparation is charged on a per sample basis and varies by type.  Run charges are based on the length of the sequencing run and whether it is single or paired end.  Prices are subject to change due to increasing reagent costs.

The next gen seq team requests all new users of our services please contact nextgen-seq@biotech.wisc.edu to set up a meeting to discuss the proposed project.


Policy Statement

All data and services provided by the University of Wisconsin Biotechnology Center Gene Expression Center are intended for research purposes only. Services are not intended nor certified for diagnostic or clinical use.