RNA Extraction Service

Can start from any sample type (tissue, cells, blood, swabs, etc…)

Semi-automated extraction using Qiagen TissueLyser

Organic extraction with cleanup using Qiagen RNAeasy kit if necessary

Specific protocol optimized for each sample type

RNA may then be used for sequencing or arrays or returned to the user for their own assays

 


Submission:

Please contact gecinfo@biotech.wisc.edu prior to sample collection if possible

The best method of sample collection is immediate flash-freezing followed by storage at -80C

RNA preservatives such as RNA Later can be used but may interfere with processing of the samples

We request 2-3 representative samples for optimization purposes

 


RNA QC:

All RNA is checked for quality on the Agilent Bioanalyzer and purity on the NanoDrop One Spectrophotometer

Results of the QC will be emailed to the user upon completion of the extraction

QC results will be approved by the user prior to continuation of any assays

 


Cost:

Sample type
   UW Rate
 External Rate
 Tissue (Skin)  Setup fee
 $505.00  $773.00
   Per sample
 $15.00  $23.00
 Cell Pellet
 Setup fee
 $250.00  $383.00
   Per sample
 $10.00  $15.00



Please contact gecinfo@biotech.wisc.edu for a quote for your project. Cost varies depending on extraction method required to extract good quality RNA from your samples.