Project step
Quality control
We will meet with you to best understand your genome editing needs
  • A unique project code is assigned and tethered to all data, reagents, and results.
  • Receive unambiguous references to your locus of interest and desired edit.
A design will be selected that maximizes specificity and efficiency to achieve your desired edit.
  • Consolidation of all annotation and design features into one software package.
  • Verification of all design components.
A single oligo including a T7 cassette, the selected target sequence, and short portion of the common gRNA sequence
  • Careful analysis of target site specificity based on empirical off-target risk parameters
  • Standardization and verification of target sequence format for oligo synthesis.
Using overlap extension PCR to synthesize a double-stranded fragment.
  • gRNA template is analyzed by gel electrophoresis and quantified by Qubit.
In vitro transcription of template generates large quantity of gRNA
  • gRNA is DNAse treated.
  • Synthesis is performed in a RNAse-free designated space.
  • In vitro transcription yield is measured with Qubit.
gRNA is column purified, precipitated and washed
  • gRNA is purified using a two-stage purification protocol.
  • gRNA integrity is confirmed by gel electrophoresis.
  • Final gRNA yield is determined with Qubit.
  • gRNA concentration is adjusted to a workable range.