How do I create a genome-edited model through UWBC?
  • How do you generated genome edits?
  • We use the CRISPR/Cas9 platform of site-directed double-stranded breaks to efficiently, reliably, and specifically generate the genome edit you wish.
  • What model organism can I use to make my genome edit?
  • We are happy to work with whatever model system best addresses your experimental needs. Most commonly we work with rodent models (mice and rats) through our synergistic relationship with the Transgenic Animal Facility. We also are developing a relationship with the Waisman Center to generate genome-edited iPS lines and a plant facility to generate genome-edited plants.
  • How can the Biotechnology Center help me make my genome edited model?
  • The Translational Genomics Facility within the UW Biotechnology Center offers a full suite of services from project conception to the delivery of a genotyped model. This includes services such as consulatation, design, target selection, target validation, reagent synthesis and purification, and genotyping.
  • How should I proceed if I'm interested in CRISPR/Cas9 services through the Translational Genomics Facility?
  • Contact Dustin Rubinstein and/or Kathy Krentz (for mouse and rat projects) for a consultation regarding your desired genome edit. If you decide you'd like to enlist our services, we will add you to our priority list.
  • If I'm not affiliated with the University of Wisconsin, can I still receive help with my CRISPR/Cas9 project?
  • Yes, we're happy to serve groups off-campus, including universities and private organizations. Please note an added 53% indirect fee for our services will be added.
  • What strains of mice and rats do you work with?
  • Most groups prefer C57BL6/J mice and Sprague-Dawley rats. However, the Transgenic Animal Facility is happy to discuss your preferred background strain. We have successfully generated CRISPR/Cas9-mediated genome edits in numerous genetic backgrounds and strains. 
  • What do I need to provide to begin my project?
  • Once you've consulted our staff, shortly you will be invited to submit your project information via this website. This includes information including PI name, institution, as well as your model organism, desired gene or locus to edit, and the nature of your desired edit. In the meantime, please email these details to Dustin Rubinstein