Genome Editing and Animal Models Core

Generation and Preservation of Genome-edited models

Genome Editing and Animal Models Core (GEAM), formerly Transgenic Animal Facility, was established in 1988 and has a long history of providing UW Madison researchers access to the latest technology for generating transgenic and gene targeted animals at a reasonable cost. They have been nationally recognized for their work in manipulating rat embryos and have been very successful in incorporating CRISPR/Cas9 technology into their program. 

GEAM provides additional related services such as embryo cryopreservation, sperm cryopreservation, IVF and strain rederivation.  Off-campus users are welcome to contact us for rates and price quotes. 

Facility staff provide the expertise, state-of-the-art laboratory space, and pathogen-free animal quarters necessary for the efficient production of transgenic and gene-edited mice and rats.
Clients may receive guidance in grant write-ups, DNA and RNA preparation for microinjection, transgenic animal husbandry, and analysis of gene-edited animals.

Recent Publications 
using CRISPR/Cas9:

Wes Pike's lab in Biochemistry
A Kidney-Specific Genetic Control Module in Mice Governs Endocrine Regulation of the Cytochrome P450 Gene Cyp27b1 Essential for Vitamin D3 Activation (August 2017).

Aki Ikeda's lab in Genetics
Mouse Tmem135 mutation reveals a mechanism involving mitochondrial dynamics that leads to age-dependent retinal pathologies (November 2016).

TALEN publication
GATA Factor-Regulated Samd14 Enhancer Confers Red Blood Cell Regeneration and Survival in Severe Anemia (August 2017). 

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Services