DNAseq is the general process of sequencing DNA and can be used for a variety of purposes. One of the most common purposes is detection of genome variants. By using enrichment methods (exome, amplicon) an investigator can target regions of interest in the genome and save considerable sequencing cost. However, some questions dictate the use of whole genome sequencing (WGS) or whole genome variant detection which entails sampling genomic DNA fragments from the entire genome to get a reasonable representation of its complete contents. Sequencing depth varies drastically depending on the project goals, organism and policy. For more details on sequencing depth see the guidelines tab.
The workflow of most DNA sequencing analysis methods start similarly and diverge at the variant calling step.
Click the link to be redirected to our: Example DNAseq report
We offer free consultations as part of the initial experimental design. We want to ensure that you have thought about all the necessary design components before you conduct your experiment. This way BRC has high quality data when it comes time for us to analyze the data. We offer this service at no charge because it is more cost effective to catch design errors before we start the analysis.
We will offer custom analysis or training at our hourly rate.
We can provide project specific analysis beyond our standard pipeline services when we are written into grants. This may be a cheaper option for labs requiring a lot of analysis time as we dedicate a percent of our effort to the project.