Genotype by Sequencing (GBS): is a molecular technique that allows genomewide genotyping of a population of organisms by performing reduced representation sequencing of their genome. GBS is a robust, simple and affordable means of SNP discovery and mapping. Users of GBS often aim to find Single Nucleotide Polymorphisms (SNPs) within a population to: promote understanding of the relationships between the organisms within, or utilize the genotyping information for genomic selection or trait association analysis.
GBS analysis is traditionally performed with large batches of samples (>=96) and the goal of determining variation within the population at a sub-selected number of sites across the genome. To reduce the genome complexity we use restriction enzymes that cleave the genomic DNA with high specificity. User beware, the choice of enzyme determines the DNA to be sequenced downstream. After cleavage the DNA is purified, adapters are ligated, a DNA sequencing library is prepared and size selected before going onto the sequencer.
Sequencing is performed using an Illumina NovaSeq 6000 to produce paired end reads (2x150 bp). Data is multiplexed en mass via sample specific barcodes that will be provided to you. If you intend to demultiplex using both reads be aware that barcodes are only present on the 5' end of Read 1 (R1, forward) which is linked to a non-barcoded Read 2 (R2, reverse) via fastq specifications.
The BRC has already analyzed GBS data for a wide variety of model and non-model organisms including:
We use the most contemporary algorithms currently available for analysis of your GBS data. These include SNP calling for your entire population via the Tassel or Stacks GBS analysis pipelines. In addition to the standard pipeline outputs we provide a customized html report containing: plate, sample and variant discovery statistics combined with population structure analyses (PCA & Phylogeny).
Click the link to be redirected to our: Example GBS Report
See our description in FAQ...
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