mRNA-Seq is a method used to sequence fragments of cDNA which are reverse-transcribed from messenger RNA (mRNA). mRNA fragments are transcribed from genes by RNA polymerase for use as a template by the ribosome to produce the protein encoded by the transcript. As such, levels of a specific mRNA are viewed as the "expression" of a gene.
A general goal of most mRNA-seq projects is to determine the expression of genes across the genome in a specific cell/tissue/organism type. Integrating gene expression information from many genes with known biochemical and genetic interactions allows generation and testing of molecular hypotheses.
One parameter essential to success in mRNA-seq is the use of adequate numbers of control and test samples. Descriptions of adequate numbers of biological and technical replicates are provided in the guidelines section.
Click the link to be directed to our: Example mRNA-Seq Report
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