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University of Wisconsin–Madison

DNA Sequencing Facility

Personnel

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Joshua R. Hyman, Ph.D., Core Director
1320 Biotechnology Center
Phone: 608-890-1934/Email: jrhyman@wisc.edu

Background: Josh has been the director of the UWBC DNA Sequencing Facility since September, 2004.  He began his career as a mechanical engineer in the defense industry, but eventually followed his passion and returned to university to pursue a degree in plant breeding and genetics, receiving his PhD in Genetics from Penn State University. His graduate and postdoctoral work focused on tomato and carrot research.


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Jeremy Niece, Research Lead
Sanger Sequencing
1322 Biotechnology Center
Phone: 608-263-9882/ Email: jjniece@wisc.edu

Background: Jeremy has been a member of the DNA Sequencing Facility for 15+. He holds a bachelor's degree in Math from the University of Wisconsin-Madison.  Jeremy's specialty is troubleshooting Sanger Sequencing and Genescan data from our ABi 3730xl sequencers.


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Justin Koshalek
1262 Biotechnology Center
Email: jakoshalek@wisc.edu

Background: Justin began working at the Next-Gen Sequencing Facility in 2010. He is a graduate of Madison Area Technical College’s Biotechnology Technician Program and the University of Wisconsin - Madison with a degree in Molecular Biology.

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James Speers
1274 Biotechnology Center
Email: jmspeers@wisc.edu

Background: James has been a member of the DNA Sequencing Facility since 2010, and specializes in 16S  metagenomic and amplicon sequencing.  

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Molly Zeller
1274 Biotechnology Center
Email: nextgen-seq@biotech.wisc.edu

Background:  Molly has been a Research Specialist at the DNA Sequencing Facility since August 2016. She received her BS at the University of Wisconsin - Oshkosh in 2010 and her MS at Colorado State University in 2015. Both degrees are in Cell and Molecular Biology. Molly helps co-manage the core with James. You will likely engage with her while inquiring about GBS, PacBio, or any Illumina sequencing projects. She enjoys playing volleyball, traveling, and hanging out with her nephew.

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Robyn Roberts
1264 Biotechnology Center
Email: nextgen-seq@biotech.wisc.edu

Background:  Robyn has been working at the DNA Sequencing Facility since March 2017 when she started as an undergraduate in the lab. Upon graduating from the University of Wisconsin – Madison with a bachelor’s degree in Biology, and a certificate in Environmental Studies, she accepted a full-time position as an Associate Research Specialist.

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Amanda Maegli
1322 Biotechnology Center
Email: nextgen-seq@biotech.wisc.edu

Background: Amanda has been a Research Specialist at the DNA Sequencing Facility since March 2015. She received her BS at the University of Wisconsin – Madison in 2010 and her MS at the University of Nebraska – Lincoln in 2013. Both degrees are in Entomology. Amanda preps gDNA, performs quality control, single nucleotide polymorphism (SNP) genotyping using KASPar assays and Microsatellite Assays, and loads MiSeq, HiSeq 2000/2500 High Throughput, and HiSeq2500 Rapid sequencers.

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Sarah Harrie 
1262 Biotechnology Center
Email: nextgen-seq@biotech.wisc.edu

Sarah has been working at the DNA Sequencing Facility since August 2018. She received her bachelor’s degree in Molecular Biology from Ferris State University and also has a Molecular Biology clinical certification from ASCP. Sarah preps gDNA, GBS, and 16s libraries as well as loads the MiSeq and NovaSeq sequencers.

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Bridget Ladell 
1264 Biotechnology Center
Email: nextgen-seq@biotech.wisc.edu

Bridget has been a Research Specialist at the DNA Sequencing Facility since August 2018. She received her BS in Molecular Biology from the University of Wisconsin-LaCrosse in 2015 and completed her MS in Environmental Engineering at the University of Illinois at Urbana-Champaign in 2018. Bridget preps High Molecular Weight DNA, GBS samples, Whole Genome Libraries, runs the Oxford Nanopore Technologies platforms, and helps process the raw data from Illumina sequencing runs. She specializes in Oxford Nanopore Technologies preps and development, chromatin conformation sequencing, and validating new sequencing methods.

Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.