Please see Table 4 for MiSeq Best Use for your project.
Table 1. MiSeq sequencing run lengths and average reads per flow cell.
Table 2. NovaSeq 6000 sequencing run lengths and average reads per flow cell.
*two day run time, all others are one day
Table 3. Read lengths available per flow cell type.
Table 4. Comparison of services that can be run on MiSeq and NovaSeq 6000.
We MUST have this information before we can proceed with your request.
Please contact firstname.lastname@example.org to arrange all sample submissions. Samples and submission forms must be submitted before a project can be entered into our queue.
Submission Forms and Guidelines
Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.
Submitting Samples/ Shipping Instructions
After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples. *We require customers to submit electronic Submission Forms!*
In Person Submission
Please give advance notice of submission date and time so staff can be prepared to receive samples. Schedule a time for drop off with a staff member at email@example.com
Please send the tracking information to:
Next Generation Sequencing: firstname.lastname@example.org
*Samples that are being shipped must have a hard copy of the submission form and guidelines in the package! This way we know what the samples are and where they are from.*
If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:
Illumina Submission Form (required with every submission) *Please note: Certain elements are not viewable on Mac Preview!
Guidelines and Sample Sheet (required with every submission)
Contact us at email@example.com with any questions.
See Illumina Sequencing Platform Policy (LINK)
All input DNA submitted after 9/12/19, along with any intermediate and/or derived products (to include undiluted stock libraries), will be stored for 6 months and then disposed of.
All customer prepared libraries submitted after 4/23/18 will be stored for 6 months and then disposed of.
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above. This surcharge is subject to change.
Illumina pricing is broken into two components: library preparation and run cost. Library preparation is charged on a per sample basis and varies by type. Run charges are based on the length of the sequencing run and whether it is single or paired end. Prices are subject to change due to increasing reagent costs.