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University of Wisconsin–Madison

DNA Sequencing Facility

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.

Oxford Nanopore Techologies

Platform Description

Oxford Nanopore Technologies offers long-read capabilities on several third-generation sequencing platforms. These platforms have the capabilities to sequence Native and Cycled DNA, cDNA, and RNA through an engineered pore.  The range of possibilities for projects on Oxford Nanopore Technologies is continually expanding at a very exciting rate and we always strive to offer the best service for research needs. We highly recommend meeting to go over your project plan so we can best assist with offering Oxford Nanopore Technologies services and create a Project Summary Plan to meet your goals.

Nanopore Service Certified

We are Certified Service Providers and run two distinct platforms to optimally fulfill your sequencing depth and coverage needs.

GridION Mk1

We use the GridION for our small to mid-sized projects. We have the flexibility to run up to 5 flow cells at a time, allowing us to work through several projects at a single time or dedicate all the space to a single sample set. We have projects generating upwards of 10Gb per flow cell and this allows us to secure the desired depth of coverage for your DNA project.


PromethION 24

The PromethION 24 allows us to significantly increase the coverage of sequencing projects. We can run 24 flow cells at a time, generating 100Gb for each. This platform is ideal for large projects, such as complete plant or human genomes.


A Flongle is a smaller sized flow cell that can be run on the GridION at a lower cost. It offers us the unique opportunity to test parameters prior to fully implementing large projects. It is also ideal for pilot projects and projects that may not require much data.


Data Analysis

The Bioinformatics Resource Center is highly skilled at handling Oxford Nanopore Technologies data. We highly recommend contacting their experts to set up a meeting and work on your project plan. 

  • Getting Started

  • New Lab or Customer Access

  • Sample Submission

Oxford Nanopore Technologies Sequencing Pricing
ONT Sequencing Pricing Table

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.

Please visit our Personnel Tab
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.