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University of Wisconsin–Madison

DNA Sequencing Facility

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.


PacBio Sequel II csp-logo_new-(1)
PacBio Seq II Photo for Platform Page

The PacBio Sequel II is a powerful, long read sequencer which utilizes Single Molecule, Real Time (SMRT) Sequencing to achieve unparalleled long read data. We are proud to be Certified Service Providers of this unique technology. The Sequel II has increased the amount of data per sequencing SMRT cell by 8X and enabled HiFi read capabilities. HiFi reads are long (10-20kb) and accurate (99.8%) – a recent HiFi run produced over 550Gb of data! This data proves to be indispensable for accurate phasing of mutations spaced more than 1 kb apart and provides genome structure on a larger scale than currently possible with short read technologies.  

 PacBio Applications 

We are happy to prepare libraries for you or accept completed libraries. For more information regarding our library prep services for PacBio sequencing click here



General Guidelines

  • Getting Started

  • New Lab or Customer Access

  • Sample Submission

PacBio Sequencing Guidelines

PacBio Pricing
Pacbio Sequencing Pricing

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.

Please visit our Personnel Tab
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.