The 3730xl Genetic Analyzer (ThermoFisher) is economical and ideal for small projects of a select number of samples needing information on small regions. The charged products labeled with dyes that are excited while migrating across the detection cell of an array can provide the sizing or sequence.
Sanger Sequencing uses a dye terminator reaction. This can be used to sequence PCR products, verification of insertion/deletion, or point mutation,
Fragment Analysis is the sizing of label PCR product with an internal ladder. Common experiments are AFLP, IDAA, RFLP and microsatellites which can be multiplexed of sizes 100-600bp.
To discuss a project or acquire a quote, please contact us! For new clients, please set up an account (see New Lab or Customer Access below). All projects must be discussed before samples are submitted.
If you would like to initiate a project or get more information, please use the following contact information, and a member of our group will get in touch with you directly.
Sanger Sequencing: email@example.com
Please contact firstname.lastname@example.org. Samples can also be dropped off in Room 1259.
Submission Forms and Guidelines
Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with samples.
Submitting Samples/Shipping Instructions
After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples.
In Person Submission
*Please give advance notice of submission date and time so staff can be prepared to receive samples.*
Please send the tracking information to:
Seal tubes and plates with the matching manufacture caps. Pad the samples to protect against the shipping abuse. Shipping on dry ice is ideal, but for most room temperature is okay. Next Morning delivery is not necessary.
If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:
UW DNA Sequencing Facility
Attn: Sanger Sequencing
425 Henry Mall
Madison WI, 53706