updated as of 9/13/2019
Please thoroughly review these policies prior to submitting samples. If you have any questions regarding this policy or any other aspect of next generation sequencing, please contact us at firstname.lastname@example.org.
Projects will not be entered into the Illumina run queue until samples and completed submission form are in the possession of the DNA Sequencing Facility. A flow cell will not be run until all lanes are filled. Upon request, UWBC will give you a tentative run date. We strive to honor this run date; however, there are issues beyond our control that may delay your run. In the event of a delay greater than a week, UWBC will email users to inform them of an updated run date. In order to expedite sequencing, please feel free to notate different acceptable run types. Typical turnaround time is 6 weeks.
If the following initial QC requirements are not met, you will be informed via email. You may elect to either resubmit samples of sufficient quality and quantity or proceed with the existing samples. Should you choose to proceed with samples that do not meet our requirements, the samples will be run AS IS, and we make no assurances on the quality of downstream data.
The UWBC strives to deliver data meeting or exceeding the following metrics:
The UWBC strives to ensure all Facility prepared libraries meet these specifications. As noted*, run success is DNA and genome dependent to a certain degree so variation occurs and some libraries will not perform as well as expected. Facility prepared libraries that grossly underperform due to Facility or instrument errors will be re-run at the earliest possible date; however, there may be a wait for a run that will meet or exceed the requested parameters.
Customer prepared libraries that do not meet these specifications can be re-run at the submitting lab’s expense.
* Approximation because there are many different factors: species, kit used, phiX %, GC content, etc. Please contact email@example.com to discuss concerns.
While we will make every effort to create successful sequencing runs with high quality data output, we cannot be responsible for improperly prepared samples. Therefore, we make no guarantees on the quantity and quality of data generated from Illumina sequencing if:
If your sequencing libraries grossly underperform, or if you have other questions/concerns regarding your data, please contact us immediately at firstname.lastname@example.org to address your concerns.
Deliverable sequence data will be stored in your download directory. These files will be kept for a limited time by the DNA Sequencing Facility but the PI is ultimately responsible for long term storage. We store the raw bcl file output from each sequencing run for approximately 30 days. If you wish to have a copy of this data, you must purchase the entire run, provide a clean external drive, and notify the DNA Sequencing Facility prior to the beginning of your sequencing run.
All input DNA submitted after 9/12/19, along with any intermediate and/or derived products (to include undiluted stock libraries), will be stored for 6 months and then disposed of.
All customer prepared libraries submitted after 4/23/18 will be stored for 6 months and then disposed of.