The DNA Sequencing Facility offers all-inclusive next generation sequencing, DNA extraction and SNP genotyping services on a rolling-submission, fee-for-service basis, with the goal of generating high-quality genomic and transcriptomic data. We work with our UW system and external collaborators to prepare DNA, RNA, ChIP, GBS, metagenomic, exome, and other sequencing libraries, provide robust sequencing data, and help interpret that data to provide meaningful biological information in a time-sensitive manner.
Read Length: 50, 150, 250 or 300 bp; single read or paired end
Run time: 6 hrs - 3 days
Data generation per lane: 1-25 million fragments sequenced in parallel; 0.5-15 Gbases data output
Ideal for: prokaryotic ChIP-seq, smallRNA-seq, small genome resequencing and targeted capture
Illumina HiSeq 2500:
Read length: 50, 100, 150 (rapid mode), or 250 (rapid mode) bp, single read or paired end
Run time: 3-6 days in standard mode, 1-3 days in rapid mode
Data generation per lane: 100-150 million fragments sequenced in parallel; 7.5-35 Gbases data output
Ideal for: de novo genome sequencing, large genome resequencing and targeted sequencing; where quick data turnaround is vital
To discuss a project or acquire a quote, please contact our NGS team. To submit samples, please review our submission guidelines and sequencing policies, fill our our sample submission form, and contact us to set up an account and coordinate sample delivery.
Please contact firstname.lastname@example.org for scheduling information. All projects must be discussed with a project manager prior to submitting samples. No projects will be scheduled until samples and sample submission forms have been dropped off to DNA Sequencing personnel in room 1250 Biotech Center.
GBS, GBS Sample List Form
gDNA, Exome, Mate Pair
Customer Prepared Libraries
Every effort is made to provide our users with data that meets Illumina performance specifications in a timely fashion. Turnaround time for Illumina sequencing is typically 4-6 weeks. For more information, please review our Illumina sequencing policy prior to submitting samples.
Pricing effective April 1, 2019
Four flowcells are available for the NovaSeq. The Illumina NovaSeq generates approximately 360M, 720M, 1845M, and 2250M reads per lane for the SP, S1, S2, and S4 flowcells respectively. Please inquire about pricing for multiple-flowcell projects.
per 2 lane flowcell**
* A NovaSeq lane requires a MiSeq nano run per lane to balance library concentrations
** Complete NovaSeq flowcells must have the same pool of libraries loaded into all lanes and require a single MiSeq nano run per flowcell to balance the library concentrations
*** This is targeted number of reads. Actual read counts may vary ±20% from targeted amounts.
Please inquire about pricing for multiple-flowcell projects.
Pricing effective March 1, 2019.
* 2x50 bp sequencing must be purchased by the flowcell
* Complete HiSeq Rapid flowcells must have the same library (or pool) loaded into both lanes
** 2x50 bp sequencing must be purchased by the flowcell
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 53% surcharge for all labor and incidentals provided; prices are as listed above. This surcharge is subject to change.
Illumina pricing is broken into two components: library preparation and run cost. Library preparation is charged on a per sample basis and varies by type. Run charges are based on the length of the sequencing run and whether it is single or paired end. Prices are subject to change due to increasing reagent costs.