Skip to main content
University of Wisconsin–Madison

DNA Sequencing Facility

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.

Amplicon - Index PCR - 20200608
Custom Amplicon

Service Description

Our sequencing specialists can provide a custom amplicon library prep service for those who would like to sequence a specific region of interest on our Illumina platforms. We are here to help you from whatever point you’re starting from; whether that is recommendations for primer design or wanting to use primers from a journal article.    

We will start with a project consultation where we will discuss optimization of a PCR reaction to amplify your region of interest and our standard amplicon library prep outlined in the chart below.  As these projects are highly customized, please contact us if you are interested in utilizing this service. 

Custom Amplicon Workflow

Expand all

Collapse all

General Guidelines

  • Getting Started

  • New Lab or Customer Access

  • Sample Submission

Custom Amplicon Submission Guidelines

  • Custom Amplicon Submission Guidelines

Library Preparation Pricing
Custom Amplicon Pricing
MiSeq Pricing (Effective 5/1/21)
MiSeq Runs Pricing Table
NovaSeq Pricing (Effective 5/1/21)
NovaSeq Runs Pricing Table

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.

Illumina pricing is broken into two components: library preparation and run cost.  Library preparation is charged on a per sample basis and varies by type.  Run charges are based on the length of the sequencing run and whether it is single or paired end.  Prices are subject to change due to increasing reagent costs.

Please visit our Personnel Tab
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.