Our sequencing specialists can provide a custom amplicon library prep service for those who would like to sequence a specific region of interest on our Illumina platforms. We are here to help you from whatever point you’re starting from; whether that is recommendations for primer design or wanting to use primers from a journal article.
We will start with a project consultation where we will discuss optimization of a PCR reaction to amplify your region of interest and our standard amplicon library prep outlined in the chart below. As these projects are highly customized, please contact us if you are interested in utilizing this service.
We MUST have this information before we can proceed with your request.
Please contact firstname.lastname@example.org to arrange all sample submissions. Samples and submission forms must be submitted before a project can be entered into our queue.
Submission Forms and Guidelines
Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.
Submitting Samples/ Shipping Instructions
After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples. *We require customers to submit electronic Submission Forms!*
In Person Submission
Please give advance notice of submission date and time so staff can be prepared to receive samples. Schedule a time for drop off with a staff member at email@example.com
Please send the tracking information to:
Next Generation Sequencing: firstname.lastname@example.org
*Samples that are being shipped must have a hard copy of the submission form and guidelines in the package! This way we know what the samples are and where they are from.*
If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:
-Follow the General Guidelines listed (See above)
-This project must be discussed with the UWBC facility prior to submission; we will provide more specific guidelines depending on your project needs
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above. This surcharge is subject to change.
Illumina pricing is broken into two components: library preparation and run cost. Library preparation is charged on a per sample basis and varies by type. Run charges are based on the length of the sequencing run and whether it is single or paired end. Prices are subject to change due to increasing reagent costs.