We provide high molecular weight (HMW) DNA extraction services for whole blood, bacteria, fungi, plant tissue, and mammalian tissue. We follow a variety of published and vetted extraction protocols dependent on the specific sample type. Not all sample types behave the same when following our tissue specific protocols and a few extraction attempts may be necessary before successfully yielding high quality, HMW DNA. We highly recommend that you schedule a meeting with our expert extraction specialists to discuss your project and specific sample type before submission.
Due to the stringency of our downstream sequencing platforms, we perform the following checks to ensure the DNA is high quality prior to working with it.
Spectrophotometry with the NanoDrop 1000 - We look for 260/280 ratios at ~1.8 and 260/230 ratios at ~2.0-2.2 to consider the DNA “pure” before continuing.
Quantification with the Quantus™ Fluorometer – We check that samples have a sufficient DNA concentration to support the next steps of the project.
Sizing with the Femto Pulse – We look for samples to be >50kb to be considered high quality, HMW DNA.
We can also work with researchers to extract HMW DNA from sample types other than those listed above and for those interested, we also offer Ultra-High Molecular Weight (UHMW) DNA Extraction services. We use UHMW DNA to secure incredibly long, continuous reads off of Oxford Nanopore Technologies Platforms. Please inquire at firstname.lastname@example.org about projects involving DNA sources not listed.
We recommend that HMW DNA is sequenced on our long-read technology that is designed specifically to work with high quality, HMW input. Learn more about Oxford Nanopore Technologies and PacBio.
You can also choose to sequence a portion of your extracted samples on short read technology. If you think this could be beneficial for your project, please check out our WGS- Short Read page for more information.
Please note: All human samples collected during and after November 2019 are considered COVID-19 pandemic specimens. If you would like DNA extracted from these types of samples please contact the facility to discuss the details of your project.
We MUST have this information before we can proceed with your request.
Please contact email@example.com to arrange all sample submissions. Samples and submission forms must be submitted before a project can be entered into our queue.
Submission Forms and Guidelines
Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.
Submitting Samples/ Shipping Instructions
After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples. *We require customers to submit electronic Submission Forms!*
In Person Submission
Please give advance notice of submission date and time so staff can be prepared to receive samples. Schedule a time for drop off with a staff member at firstname.lastname@example.org
Please send the tracking information to:
Next Generation Sequencing: email@example.com
*Samples that are being shipped must have a hard copy of the submission form and guidelines in the package! This way we know what the samples are and where they are from.*
If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below: