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University of Wisconsin–Madison

DNA Sequencing Facility

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.

Extractions - High Throughput - 20200608

High Molecular Weight Extraction

Service Description 

We provide high molecular weight (HMW) DNA extraction services for whole blood, bacteria, fungi, plant tissue, and mammalian tissue. We follow a variety of published and vetted extraction protocols dependent on the specific sample type. Not all sample types behave the same when following our tissue specific protocols and a few extraction attempts may be necessary before successfully yielding high quality, HMW DNA. We highly recommend that you schedule a meeting with our expert extraction specialists to discuss your project and specific sample type before submission. 

Due to the stringency of our downstream sequencing platforms, we perform the following checks to ensure the DNA is high quality prior to working with it.   

    1. Spectrophotometry with the NanoDrop 1000 - We look for 260/280 ratios at ~1.8 and 260/230 ratios at ~2.0-2.2 to consider the DNA “pure” before continuing. 

    2. Quantification with the Quantus™ Fluorometer – We check that samples have a sufficient DNA concentration to support the next steps of the project.

    3. Sizing with the Femto Pulse – We look for samples to be >50kb to be considered high quality, HMW DNA. 

    We can also work with researchers to extract HMW DNA from sample types other than those listed above and for those interested, we also offer Ultra-High Molecular Weight (UHMW) DNA Extraction services. We use UHMW DNA to secure incredibly long, continuous reads off of Oxford Nanopore Technologies Platforms. Please inquire at nextgen-seq@biotech.wisc.edu about projects involving DNA sources not listed.

    Sequencing Recommendations  

    We recommend that HMW DNA is sequenced on our long-read technology that is designed specifically to work with high quality, HMW input. Learn more about Oxford Nanopore Technologies and PacBio

    You can also choose to sequence a portion of your extracted samples on short read technology. If you think this could be beneficial for your project, please check out our WGS- Short Read page for more information. 

    Please note: All human samples collected during and after November 2019 are considered COVID-19 pandemic specimens. If you would like DNA extracted from these types of samples please contact the facility to discuss the details of your project.

    General Guidelines

    • Getting Started

    • New Lab or Customer Access

    • Sample Submission

    Extraction Pricing
    HMW Extraction Pricing
    EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.
    Please visit our Personnel Tab
    Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.