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University of Wisconsin–Madison

DNA Sequencing Facility

We are open for business! We are happy to be back. During Phase 1, we will be operating at reduced capacity. We will make every effort to keep turnaround times short as we understand there are many projects that have been waiting. We appreciate your patience and business. The UW Biotechnology Center is following University guidelines regarding COVID-19.

Please refer to the side panel for our current sample submission process.

Exome

Exome Capture

Service Description
Exome sequencing allows researchers to capture the exons, also known as the coding regions, within the genome. This type of library preparation is possible with various types of samples including human, non-human, and formalin-fixed paraffin embedded (FFPE) DNA.

Advantages
By pooling pre-capture libraries prior to capture, we reduce the total number of captures performed, which decreases costs significantly. We have performed an evaluation of this method, pooling 12 samples per capture, and found all samples to have acceptable data quality. The number of pre capture libraries pooled is flexible depending on your project goals and coverage requirements. Pooling pre-capture libraries is what we currently offer as our standard exome service; though, we are happy to accommodate the needs of your specific project.  Please inquire for more information about our exome services.    
 
Exome Workflow
Exome Sequencing vs. Whole Genome Sequencing
While exome sequencing is only sequencing the exons, whole genome sequencing (WGS) includes everything in the genome: introns (non-coding), exons (coding), and mitochondrial DNA. WGS can result in a lot of data to sort through and may include irrelevant information. Exome sequencing allows researchers to narrow in on a subset or specific gene regions within the exome. 

General Guidelines

  • Getting Started

  • New Lab or Customer Access

  • Sample Submission

Please contact us at nextgen-seq@biotech.wisc.edu for exome capture pricing!
Please visit our Personnel Tab
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.