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University of Wisconsin–Madison

DNA Sequencing Facility

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.

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Fragment Analysis

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Service Description 

As part of our Sanger Service offerings, we have a variety of fragment analysis services to help with sizing of fluorescently labeled products of 100-600bp. You run the assays in your lab and can bring them over as “Ready to Go” or we can add the ladder before running.

FA Custom Assays

Generation of labeled fragments 

You will run all the PCR reactions in your lab prior to submission. We encourage you to run the reaction out on an agarose gel before submitting to ensure the existence and rough concentration of the products. 

Fluorophores 

The capillary instrumentation is spectrally calibrated to detect various fluorophores within filter sets D or G5. 

Dye
Color
Dye Set D
Dye Set G5
 6-FAM Blue Product
Product
HEX/VIC Green Product Product
NED
Yellow
Product
Product
ROX
Red
Ladder
Product
LIZ
Orange
N/A
Ladder

You can pool products by different colors and sizes, but each well must have an internal molecular weight (ladder) and the entire plate can only be a single dye set. For custom sized products, 6-FAM labeled primers are a good place to start because that label is the most robust (the brightest, and most stable) and can be made cheaply.
Sanger Sequencing Guidelines

  • Getting Started

  • New Lab or Customer Access

  • Sanger Sequencing Submission Guidelines

Fragment Analysis Pricing
fragment analysis pricing
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55.5% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.
Please visit our Personnel Tab
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.