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University of Wisconsin–Madison

DNA Sequencing Facility

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.

GBS

GBS

Service Description 

GBS (Genotype By Sequencing) is a cost effective, high throughput service that helps in the discovery of single nucleotide polymorphisms (SNP) for plant and animals along with genotyping studies. If your species does not have a known enzyme combination for GBS, we offer optimization/restriction enzyme selection! We have also improved our GBS prep by adding a post-PCR normalization step.  

    GBS Fast Facts



    *The University of Wisconsin-Madison Biotechnology Center

    – DNA Sequencing Facility is a Licensed Service Provider, KG logo klein
    for internal and external clients, providing GBS services under license from Keygene N.V. which owns patents and patent applications protecting its Sequence Based Genotyping technologies. 

    **KeyGene license fee will be applied to any client-prepared libraries   


    Optimization of Species 

    Species' genomes respond differently to combinations of restriction enzymes, this can all be impacted by the G/C content, methylation status, or complexity amongst other conditions. If the enzyme combination is not known yet, species will need to have optimization/restriction enzyme selection performed to determine which enzymes to proceed with for GBS. We have worked with a variety of different species and are open to working with new species. Please contact us at nextgen-seq@biotech.wisc.edu to discuss your project. 

    Advantages 

    We have updated our GBS prep protocol to now include post-PCR Normalization! Post-PCR normalization provides data with a more even distribution of reads across a 96 well plate. This ensures that low concentration samples get even coverage. A comparison of the number of reads per GBS samples with or without post-PCR normalization can be seen in two different plates of samples in Figure 1 and 2.

    GBS Data Figures 1 & 2

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    General Guidelines

    • Getting Started

    • New Lab or Customer Access

    • Sample Submission

    GBS Submission Guidelines

    • Submission Forms and Guidelines

    • GBS Submission Guidelines

    • Policies

    Library Preparation Pricing
    GBS Pricing
    MiSeq Pricing (Effective 5/1/21)
    MiSeq Runs Pricing Table
    NovaSeq Pricing (Effective 5/1/21)
    NovaSeq Runs Pricing Table

    EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.

    Illumina pricing is broken into two components: library preparation and run cost.  Library preparation is charged on a per sample basis and varies by type.  Run charges are based on the length of the sequencing run and whether it is single or paired end.  Prices are subject to change due to increasing reagent costs.

    Please visit our Personnel Tab
    Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.