GBS (Genotype By Sequencing) is a cost effective, high throughput service that helps in the discovery of single nucleotide polymorphisms (SNP) for plant and animals along with genotyping studies. If your species does not have a known enzyme combination for GBS, we offer optimization/restriction enzyme selection! We have also improved our GBS prep by adding a post-PCR normalization step.
*The University of Wisconsin-Madison Biotechnology Center
– DNA Sequencing Facility is a Licensed Service Provider,
for internal and external clients, providing GBS services under license from Keygene N.V. which owns patents and patent applications protecting its Sequence Based Genotyping technologies.
**KeyGene license fee will be applied to any client-prepared libraries
Optimization of Species
Species' genomes respond differently to combinations of restriction enzymes, this can all be impacted by the G/C content, methylation status, or complexity amongst other conditions. If the enzyme combination is not known yet, species will need to have optimization/restriction enzyme selection performed to determine which enzymes to proceed with for GBS. We have worked with a variety of different species and are open to working with new species. Please contact us at firstname.lastname@example.org to discuss your project.
We have updated our GBS prep protocol to now include post-PCR Normalization! Post-PCR normalization provides data with a more even distribution of reads across a 96 well plate. This ensures that low concentration samples get even coverage. A comparison of the number of reads per GBS samples with or without post-PCR normalization can be seen in two different plates of samples in Figure 1 and 2.
We MUST have this information before we can proceed with your request.
Please contact email@example.com to arrange all sample submissions. Samples and submission forms must be submitted before a project can be entered into our queue.
Submission Forms and Guidelines
Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.
Submitting Samples/ Shipping Instructions
After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples. *We require customers to submit electronic Submission Forms!*
In Person Submission
Please give advance notice of submission date and time so staff can be prepared to receive samples. Schedule a time for drop off with a staff member at firstname.lastname@example.org
Please send the tracking information to:
Next Generation Sequencing: email@example.com
*Samples that are being shipped must have a hard copy of the submission form and guidelines in the package! This way we know what the samples are and where they are from.*
If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:
Illumina Submission Form (required with every submission) *Please note: Certain elements are not viewable on Mac Preview!
Guidelines and Sample Sheet (required with every submission)
Follow general guidelines listed (See Next Generation Sequencing General Guidelines above).
Success in GBS is determined primarily by the quantity and quality of the submitted DNA. Please submit the following quality control metrics with EACH project.
Lyophilized DNA is also acceptable, but we would prefer to have DNA suspended in 10 mM Tris or nuclease-free water. Please avoid EDTA.
Figure 1. Gel image example for gDNA and HindII Digest (Gel Image Provided by Mockler Lab).
Contact us at firstname.lastname@example.org with any questions.
See Illumina Sequencing Platform Policy (LINK)
All input DNA submitted after 9/12/19, along with any intermediate and/or derived products (to include undiluted stock libraries), will be stored for 6 months and then disposed of.
All customer prepared libraries submitted after 4/23/18 will be stored for 6 months and then disposed of.
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above. This surcharge is subject to change.
Illumina pricing is broken into two components: library preparation and run cost. Library preparation is charged on a per sample basis and varies by type. Run charges are based on the length of the sequencing run and whether it is single or paired end. Prices are subject to change due to increasing reagent costs.