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University of Wisconsin–Madison

DNA Sequencing Facility

We are open for business! We are happy to be back. During Phase 1, we will be operating at reduced capacity. We will make every effort to keep turnaround times short as we understand there are many projects that have been waiting. We appreciate your patience and business. The UW Biotechnology Center is following University guidelines regarding COVID-19.

Please refer to the side panel for our current sample submission process.

ONT Updated
Oxford Nanopore Technologies Library Preps

Service Description Nanopore Service Certified

We are a certified Oxford Nanopore Technologies provider with years of experience helping clients use Third-Generation Sequencing techniques to achieve their research goals. 

Oxford Nanopore Technologies developed unique platforms that allow for long reads of contiguous DNA strands. These platforms use flow cells with pore and membrane technology to read strands of DNA by base associated electrical signal.  

We operate a Nanopore GridION Mk1 which runs 5 flow cells at a time and generating upwards of 10Gb of data per flow cell. We have recently purchased a PromethION 24, capable of running 24 flow cells at a time and generating on average, more than 100 Gb of data per flow cell. PromethION services are currently in evaluation.

Advantages 

Oxford Nanopore Technologies library prep kits allow us to sequence native DNA, eliminating PCR bias from the data. Native DNA in sequencing maintains strand methylation status. The pore-based flow cells allow for superior reading through long repeat regions compared to traditional next-generation sequencing. These long reads are significant for detecting structural variants throughout the genome. Past projects have consistently generated strands upwards of 100kb (kilobases).

Recommendations 

To best take advantage of this sequencing method, we recommend that you submit very high-quality DNA. We offer High Molecular Weight Extraction services. We have successfully extracted High Molecular and Ultra High Molecular Weight DNA from a wide variety of microorganisms, plants, and animals. 

To complement our Oxford Nanopore Technologies services, we offer PacBio HiFi and we have had great success with projects that use this service performed in tandem with Oxford Nanopore Technologies to fully elucidate long stretches of genomes. 

Many projects require special upstream attention and handling before sequencing on the GridION or PromethION; we would love to meet with you and go over your project plan so we can best assist with offering Oxford Nanopore Technologies services and create a Project Summary Plan to meet your goals.

General Guidelines

  • Getting Started

  • New Lab or Customer Access

  • Sample Submission

Library Preparation and Sequencing Pricing
Oxford Nanopore Technologies Pricing
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.
Please visit our Personnel Tab
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.