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University of Wisconsin–Madison

DNA Sequencing Facility

We are open for business! We are happy to be back. During Phase 1, we will be operating at reduced capacity. We will make every effort to keep turnaround times short as we understand there are many projects that have been waiting. We appreciate your patience and business. The UW Biotechnology Center is following University guidelines regarding COVID-19.

Please refer to the side panel for our current sample submission process.

Pacbio 20200601 -2

PacBio Libraries

Service Description

PacBio CSP

The PacBio Sequel II is a powerful long read sequencer that can provide high quality, long read data to achieve a wide variety of research goals. We offer many different PacBio library preps to meet your specific needs. Our long-read sequencing projects are tailored to meet your needs – please contact us about your project and research goals so we can offer consultation and more specific advice depending on your project.

PacBio Services Final 20200609


Recommendations 

To complement our PacBio services, we offer Oxford Nanopore Technologies library prep and sequencing. We have had great success with projects that use this service performed in tandem with PacBio to fully elucidate long stretches of genomes. 

We offer high molecular weight (HMW) extraction if you need assistance in obtaining high quality HMW DNA. Clean, high quality, HMW DNA is essential to the success of library prep and sequencing on the PacBio system.  The mass of DNA required depends on the type of prep you would like us to perform, which can be a large amount in some instances. 

General Guidelines

  • Getting Started

  • New Lab or Customer Access

  • Sample Submission

PacBio Pricing
Pacbio Sequencing Pricing
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.
Please visit our Personnel Tab
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.