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University of Wisconsin–Madison

DNA Sequencing Facility

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.

QC Final

Quality Control and Customer Prepared Libraries

Service Description 

We offer quality control services that include initial QC for library development, size selection, and final QC of customer prepared libraries that are ready to go for sequencing. Our scientists have years of experience analyzing libraries and troubleshooting issues. We use state-of-the-art equipment for quantification and sizing of DNA, from initial library input to finished library. Accurate QC ensures high quality libraries are run on the sequencers at the same concentration and helps identify adapter/primer dimers and unintended products that may cause sequencing issues to arise. We also offer automated size selection prior to or after library preparation, which allows for precise, discrete selection of DNA.

QC Service Options

Sample Sizing
SPRI Purification
Size Selection

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General Guidelines

  • Getting Started

  • New Lab or Customer Access

  • Sample Submission

Quality Control and Customer Submitted Guidelines

Library QC Pricing
Customer Prepared Library Pricing Table
MiSeq Pricing (Effective 5/1/21)
MiSeq Runs Pricing Table
NovaSeq Pricing (Effective 5/1/21)
NovaSeq Runs Pricing Table

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.

Illumina pricing is broken into two components: library preparation (or QC) and run cost.  Library preparation is charged on a per sample basis and varies by type.  Run charges are based on the length of the sequencing run and whether it is single or paired end.  Prices are subject to change due to increasing reagent costs.

Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.