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University of Wisconsin–Madison

DNA Sequencing Facility

We are open for business! We are happy to be back. During Phase 1, we will be operating at reduced capacity. We will make every effort to keep turnaround times short as we understand there are many projects that have been waiting. We appreciate your patience and business. The UW Biotechnology Center is following University guidelines regarding COVID-19.

Please refer to the side panel for our current sample submission process.

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Sanger Sequencing

Service Description 

We offer a variety of cost-effective, high-throughput Sanger Sequencing options. This is still the gold standard for DNA Sequencing. 

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Quick Sequencing 

The most economical service involves setting up the reaction in your own lab, then submitting the amplified products to our facility. We can go over protocols with you and provide the sequencing reagent, at a discounted rate, to carry out the reactions in your laboratory (a PCR machine is the only required equipment). If you complete the reactions in your lab, but you would like the unincorporated dyes removed, we can purify the products using SPRI beads.

Full Service 

We also offer expert service in sequencing if you wish to provide the template DNA and primer. Every effort is made to provide at least 600 bp of excellent sequence (Q score >20), though template composition and purity are crucial determinants of quality. Strategies are available to sequence difficult GC rich templates or homopolymer regions; please indicate if such regions are present.

Plate Options 

Different options are available for the processing of 96 well V-bottom plates for either Quick Service or Full Service. Please consult the price list to see what we can do for you if samples are submitted in plates.

Custom 

We can also carry out larger sequencing projects involving editing and assembly. Price is based on the degree of confidence required (i.e., single strand vs. double strand, one vs. multiple passes); contact us for more information, dnaseq@biotech.wisc.edu.

Sanger Sequencing Guidelines

  • Getting Started

  • New Lab or Customer Access

  • Sanger Sequencing Submission Guidelines

Sanger Sequencing Pricing
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.
Please visit our Personnel Tab
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.