We offer a variety of cost-effective, high-throughput Sanger Sequencing options. This is still the gold standard for DNA Sequencing.
The most economical service involves setting up the reaction in your own lab, then submitting the amplified products to our facility. We can go over protocols with you and provide the sequencing reagent, at a discounted rate, to carry out the reactions in your laboratory (a PCR machine is the only required equipment). If you complete the reactions in your lab, but you would like the unincorporated dyes removed, we can purify the products using SPRI beads.
We also offer expert service in sequencing if you wish to provide the template DNA and primer. Every effort is made to provide at least 600 bp of excellent sequence (Q score >20), though template composition and purity are crucial determinants of quality. Strategies are available to sequence difficult GC rich templates or homopolymer regions; please indicate if such regions are present.
Different options are available for the processing of 96 well V-bottom plates for either Quick Service or Full Service. Please consult the price list to see what we can do for you if samples are submitted in plates.
We can also carry out larger sequencing projects involving editing and assembly. Price is based on the degree of confidence required (i.e., single strand vs. double strand, one vs. multiple passes); contact us for more information, email@example.com.
To discuss a project or acquire a quote, please contact us! For new clients, please set up an account (see New Lab or Customer Access below). All projects must be discussed before samples are submitted.
If you would like to initiate a project or get more information, please use the following contact information, and a member of our group will get in touch with you directly.
Sanger Sequencing: firstname.lastname@example.org
Please contact email@example.com. Samples can also be dropped off in Room 1259.
Submission Forms and Guidelines
Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with samples.
Submitting Samples/Shipping Instructions
After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples.
In Person Submission
*Please give advance notice of submission date and time so staff can be prepared to receive samples.*
Please send the tracking information to:
Seal tubes and plates with the matching manufacture caps. Pad the samples to protect against the shipping abuse. Shipping on dry ice is ideal, but for most room temperature is okay. Next Morning delivery is not necessary.
If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:
UW DNA Sequencing Facility
Attn: Sanger Sequencing
425 Henry Mall
Madison WI, 53706