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University of Wisconsin–Madison

DNA Sequencing Facility

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.

WGS -Short Read 20200608

Long Read Sequencing

Service Description 

Our long read whole genome sequencing (WGS) service utilizes Oxford Nanopore Technologies and Pacific Biosciences (PacBio) platforms to characterize your novel genome of interest or re-characterize organisms with known genomes.  Our team has extensive experience with a variety of sample types – we are continuously attempting new preps and sequencing protocols to push the boundaries of what these platforms can offer.   

General Project Progression

Long Read WGS Flowchart Final 20200609


De novo genome assembly  

Our de novo genome assembly approach uses both ultra-long Oxford Nanopore Technologies reads alongside PacBio HiFi data. The ultra-long Oxford Nanopore Technologies reads are upwards of 100kb and the HiFi reads are 10-20kb in length with 99.8% accuracy; the combination of these two technologies results in extremely powerful data to create high quality genome assemblies.  De novo genome assembly projects are tailored to your needs and depend on organism type and genome size; please contact us if you are interested in this service.  If you require bioinformatic analysis, our group is highly qualified and experienced with these types of projects – please contact the BRC here.  

Recommendations  

Long read sequencing, requires extremely clean high molecular weight (HMW) DNA. This can be challenging for some organism types. Please see our HMW Extraction page if this service would benefit your needs. 

General Guidelines

  • Getting Started

  • New Lab or Customer Access

  • Sample Submission

Oxford Nanopore Technologies Pricing
Oxford Nanopore Technologies Pricing
PacBio Sequencing Pricing
Pacbio Sequencing Pricing
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.
Please visit our Personnel Tab
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.