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University of Wisconsin–Madison

DNA Sequencing Facility

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.

WGS -Long Read 20200608

Short Read Sequencing

Service Description 

Our Whole genome sequencing (WGS) service provides a broad overview of the entire genome. This type of sequencing only requires 10ng of input DNA and allows researchers to explore the genome for both small and large variants. WGS can be done on a multitude of organisms such as human, bacteria, fungi, and plants as well as metagenomes. Depending on the sequencing required for the project, WGS libraries can be run on either Illumina’s MiSeq or NovaSeq 6000.

Short Read WGS Flowchart

Genome Assembly 

Whole genome sequencing can be used for reference based sequencing or assembly, the comparison of new sequencing to a known reference sequencing. Conversely, whole genome sequencing is also ideal for discovering novel genomes because it offers a large amount of data to help characterize/identify any species. We recommend combining long read and short read sequencing to create a comprehensive genome assembly. Check out the Whole Genome Sequencing – Long Read page for more information. 

WGS Long Read

Metagenomic Sequencing 

Metagenomic sequencing allows researchers to analyze all microbial genomes present within one sample collection. It allows researchers to study microbial genomes that otherwise can’t be cultivated. This method can be performed using DNA from various types of environmental samples such as air, soil, aquatic sediment, microbiome, city (subways), and marine. If you are interested in specifically targeting the bacteria, fungi, or eukaryotic organisms within an environmental sample, then please check out the 16s/18s/ITS Amplicon Sequencing page

General Guidelines

  • Getting Started

  • New Lab or Customer Access

  • Sample Submission

WGS Short Read Submission Guidelines

  • Submission Forms and Guidelines

  • Genomic DNA Submission Guidelines

Library Preparation Pricing
WGS-Short Read Pricing
MiSeq Pricing (Effective 5/1/21)
MiSeq Runs Pricing Table
NovaSeq Pricing (Effective 5/1/21)
NovaSeq Runs Pricing Table

EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above.  This surcharge is subject to change.

Illumina pricing is broken into two components: library preparation and run cost.  Library preparation is charged on a per sample basis and varies by type.  Run charges are based on the length of the sequencing run and whether it is single or paired end.  Prices are subject to change due to increasing reagent costs.

Please visit our Personnel Tab
Unless otherwise specified, all data and reagents distributed by the University of Wisconsin Biotechnology Center DNA Sequencing Facility are intended for research purposes only. They are not intended nor certified for diagnostic or clinical use.  Clinical services are provided through our collaboration with the UW Collaborative Genomics Core.