Whole genome sequencing can be used for reference based sequencing or assembly, the comparison of new sequencing to a known reference sequencing. Conversely, whole genome sequencing is also ideal for discovering novel genomes because it offers a large amount of data to help characterize/identify any species. We recommend combining long read and short read sequencing to create a comprehensive genome assembly. Check out the Whole Genome Sequencing – Long Read page for more information.
Metagenomic sequencing allows researchers to analyze all microbial genomes present within one sample collection. It allows researchers to study microbial genomes that otherwise can’t be cultivated. This method can be performed using DNA from various types of environmental samples such as air, soil, aquatic sediment, microbiome, city (subways), and marine. If you are interested in specifically targeting the bacteria, fungi, or eukaryotic organisms within an environmental sample, then please check out the 16s/18s/ITS Amplicon Sequencing page
We MUST have this information before we can proceed with your request.
Please contact firstname.lastname@example.org to arrange all sample submissions. Samples and submission forms must be submitted before a project can be entered into our queue.
Submission Forms and Guidelines
Please check the service page guidelines for specific Submission Forms and Guidelines that need to be included with your samples.
Submitting Samples/ Shipping Instructions
After setting up a customer account, filling out submission form, and reviewing submission guidelines, you can now submit or send samples. *We require customers to submit electronic Submission Forms!*
In Person Submission
Please give advance notice of submission date and time so staff can be prepared to receive samples. Schedule a time for drop off with a staff member at email@example.com
Please send the tracking information to:
Next Generation Sequencing: firstname.lastname@example.org
*Samples that are being shipped must have a hard copy of the submission form and guidelines in the package! This way we know what the samples are and where they are from.*
If shipping samples from outside UWBC, ship via express shipping carrier on dry ice to the address below:
Illumina Submission Form (required with every submission) *Please note: Certain elements are not viewable on Mac Preview!
Guidelines and Sample Sheet (required with every submission)
Follow the General Guidelines listed (See Above)
-If DNA was quantified using a fluorometric assay (Qubit, Picogreen), please provide 100-500ng of DNA*
-If DNA was quantified using spectrophotometric means (NanoDrop), please provide 500ng-1ug of DNA*
DNA should be clean and free of inhibitors. A good indicator of quality DNA is a NanoDrop reading of 260/280 >1.8 and 260/230 >2.0
Please submit at least 15uL of DNA
*the prep requires 10-100ng input DNA
EXTERNAL RATES: Clients not affiliated with UW Madison or the University of Wisconsin system are charged a 55% surcharge for all labor and incidentals provided; prices are as listed above. This surcharge is subject to change.
Illumina pricing is broken into two components: library preparation and run cost. Library preparation is charged on a per sample basis and varies by type. Run charges are based on the length of the sequencing run and whether it is single or paired end. Prices are subject to change due to increasing reagent costs.