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University of Wisconsin–Madison

Genome Editing & Animal Models

Now encompassing the previous Transgenic Animal Facility

To our prospective clients - UW Biotechnology Center staff are currently working at home until further notice per University guidelines regarding COVID-19. We ask that you refrain from sending or dropping off samples until further notice. We apologize for the delay in service.
To our current clients - Thank you for your patience as we work to complete your projects. Expect delays of 2 or more weeks as we follow University guidelines regarding COVID-19.
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See a presentation by the directors of this facility here: Bio-Tech Talks

GEAM provides support for the generation of novel CRISPR models and for the cryopreservation, rederivation, and maintenance of animal models. A full suite of services is available, including:

  • Generation of mouse and rat CRISPR models

  • Cryopreservation of mouse and rat embryos and sperm

  • Rederivation of rodent animal models

  • Generation of transgenic and ES cell targeted models 

  • Support for CRISPR cell lines and other models

Some common services

TGF_consultarrowDesign_and_Synthesisarrowmicroinject arrowGenotyping_sangerarrowCryo_2

After consultation with the investigator, CRISPR project design and reagent synthesis is completed. Reagents are injected into rodent embryos, and resultant pups are genotyped. The line can be returned to the investigator while also cryopreserved.

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Lines can be recovered from cryopreserved sperm, embryos, or ES cells.

GEAM history

Genome Editing and Animal Models Core (GEAM), formerly Transgenic Animal Facility, was established in 1988 and has a long history of providing UW Madison researchers access to the latest technology for generating transgenic and gene targeted animals at a reasonable cost. We have been nationally recognized for our work in manipulating rat embryos and have been very successful in incorporating CRISPR/Cas9 technology into our program.

GEAM is a Shared Resource of the University of Wisconsin-Madison Carbone Cancer Center.