Now encompassing the previous Transgenic Animal Facility
GEAM provides support for the generation of novel CRISPR models and for the cryopreservation, rederivation, and maintenance of animal models. A full suite of services is available, including:
Generation of mouse and rat CRISPR models
Cryopreservation of mouse and rat embryos and sperm
Rederivation of rodent animal models
Generation of transgenic and ES cell targeted models
Support for CRISPR cell lines and other models
After consultation with the investigator, CRISPR project design and reagent synthesis is completed. Reagents are injected into rodent embryos, and resultant pups are genotyped. The line can be returned to the investigator while also cryopreserved.
Lines can be recovered from cryopreserved sperm, embryos, or ES cells.
Genome Editing and Animal Models Core (GEAM), formerly Transgenic Animal Facility, was established in 1988 and has a long history of providing UW Madison researchers access to the latest technology for generating transgenic and gene targeted animals at a reasonable cost. We have been nationally recognized for our work in manipulating rat embryos and have been very successful in incorporating CRISPR/Cas9 technology into our program.