Comprehensive CRISPR/Cas9 services available

GEAM is happy to provide comprehensive support for your CRISPR/Cas9 research project. We will share our extensive CRISPR/Cas9 experience to guide your project from conception to the creation of your desired genome-edited model.

GEAM interfaces with UW-Madison facilities to generate your genome edited model. We have routinely and successfully generated many types of genome edits in many models, and we can provide genome-edited mice and rats, iPS cells, and plants.

We will help you build your desired model of any species, but we specialize in the generation of mouse and rat CRISPR models.

Consultation

GEAM can help you plan your genome editing project. Whether you are an experienced user of genome editing strategies or you simply have an idea that would allow you to ideally address your experimental questions, GEAM is happy to provide ideas and suggestions on strategies and best-practices.

Design

GEAM staff has extensive experience genome-editing projects. Any one of many design pitfalls can seriously imperil a genome editing project, and we are happy to help guide you through this critical stage. We have designed numerous mouse, rat, Drosophila, and cell-line genome editing projects.

Reagent synthesis

We have implemented a highly efficient, synthesis-based method for generating gRNA. We reliably generate purified, injection-grade gRNAs for rodent embryo microinjection. We also synthesize gRNA expression vectors, donor constructs, single-stranded donor molecules, and other reagents for genome editing projects.

Microinjection of embryos to produce gene-edits in mice and rats

After GEAM synthesizes and purifies the necessary reagents, Kathy Krentz will schedule a microinjection day. The gRNA, Cas9, ssODN, and/or plasmid DNA (if applicable) will be microinjected into fertilized one-cell embryos. The injected one-cell embryos are then implanted into the pseudopregnant recipients and offspring are born 19 days (mice) or 22 days (rats) later. At weaning, animals are genotyped to detect presence of edits.  Founders can be bred within the Biotechnology Center or shipped to the investigators for breeding. 

Genotyping

We provide a number of genotyping solutions most appropriate for your model of choice. We offer high-throughput cell line screening for CRISPR/Cas9 generated knock-out projects. We also regularly genotype F1 litters for mouse and rat models.


 Instead of 1.5-2 years to make a genome edited mouse, it now
takes as little as 4 months!

 Some examples of modifications GEAM has successfully created:

  • Gene knockout
  • SNP Generation
  • Precise excisions
  • Conditional allele
  • Reporter Generation
  • Tag insertion
  • Cre knock-in