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University of Wisconsin–Madison

Genome Editing & Animal Models

Embryo Cryopreservation

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.
Sperm vs Embryo cryo chart  
  • Embryo cryopreservation is an efficient means of long-term storage of valuable strains.
  • Embryo cryopreservation works well for double and triple gene mutations on standard genetic backgrounds.
  • This service is available for both mice and rats.
  • Cheaper and easier recovery to live offspring.
  • Yearly storage fee of $95. 

Contact Kathy Krentz for details about rat embryo cryopreservation.


Mouse embryo cryopreservation has the following steps: 

  1. Contact Kathy Krentz (kjkrentz@wisc.edu) to discuss scheduling.
  2. Assessment of animal colony to assure one proven male and at least 8 weaned females are available for superovulation. GEAM may need close to 15 females to obtain good quality embryos for cryopreservation. 
  3. GEAM will perform IVF and cryopreserve two-cell embryos. 
  4. We recommend freezing 100-125 embryos if homozygous or 175-200 heterozygous embryos are preserved. 
  5. Recovery of live animals by thawing and surgically transferring the frozen embryos into pseudopregant recipients. 

 Mouse Embryo Cryopreservation    UW Price 
 External Price 
 1-5 donor females   $880  
 6-10 donor females   $1060  
 11-14 donor females   $1200