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University of Wisconsin–Madison

Genome Editing & Animal Models

Embryo Cryopreservation

We are open for business! We are happy to be back. During Phase 1, we will be operating at reduced capacity. We will make every effort to keep turnaround times short as we understand there are many projects that have been waiting. We appreciate your patience and business. The UW Biotechnology Center is following University guidelines regarding COVID-19.
Sperm vs Embryo cryo chart  
  • Embryo cryopreservation is an efficient means of long-term storage of valuable strains.
  • Embryo cryopreservation works well for double and triple gene mutations on standard genetic backgrounds.
  • This service is available for both mice and rats.
  • Cheaper and easier recovery to live offspring.
  • Yearly storage fee of $95. 

Contact Kathy Krentz for details about rat embryo cryopreservation.


Mouse embryo cryopreservation has the following steps: 

  1. Contact Kathy Krentz (kjkrentz@wisc.edu) to discuss scheduling.
  2. Assessment of animal colony to assure one proven male and at least 8 weaned females are available for superovulation. GEAM may need close to 15 females to obtain good quality embryos for cryopreservation. 
  3. GEAM will perform IVF and cryopreserve two-cell embryos. 
  4. We recommend freezing 100-125 embryos if homozygous or 175-200 heterozygous embryos are preserved. 
  5. Recovery of live animals by thawing and surgically transferring the frozen embryos into pseudopregant recipients. 

 Mouse Embryo Cryopreservation    UW Price 
 External Price 
 1-5 donor females   $880  
 6-10 donor females   $1060  
 11-14 donor females   $1200