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University of Wisconsin–Madison

Genome Editing & Animal Models

In Vitro Fertilization (IVF)

B6 IVF
  • In vitro fertilization (IVF) is a form of assisted reproduction technology to recover lines from frozen sperm
  • IVF can be used to rescue lines that have stopped breeding. 
  • Please contact Kathy Krentz to discuss details.  If you plan to obtain frozen sperm from a repository, please have frozen samples shipped directly to Kathy.
  1. Oocytes: Labs need to provide 8-10 young (21-27 day old females) for superovulation. These can be colony raised or purchased through vendors. All females are delivered to the Biotechnology Center and GEAM will superovulate them. 
  2. Fresh sperm: For lines that need rescuing, one male breeder (preferred) is delivered to GEAM on the morning of the IVF. GEAM will sacrifice him and assess sperm quality. If motility is >25%, sperm is used for IVF and excess cryopreserved if the lab requests.
  3. Frozen sperm: Sperm will be thawed via instructions from the repository. If the instructions suggest using media not typically used, GEAM will make the solution and charge the investigator ($110/hr). Frozen samples are thawed on the morning of the IVF. 

Disclaimer: The success of the IVF is dependent on the mouse strain, sperm motility, sperm counts, post-thaw motility and egg quality.

IN VITRO FERTILIZATION WITH EMBRYO TRANSFER (MOUSE)

UW PRICE

EXTERNAL PRICE

Plus the cost of 10 females for oocyte production

 

 

IVF + transfer into one recipient mom

$880

$1,346.40

IVF + transfer into two recipient mom

$970

$1,484.10

IVF + transfer into three recipient mom

$1,060

$1,621.80

 

IN VITRO FERTILIZATION WITH EMBRYO CRYOPRESERVATION (MOUSE)

UW PRICE

EXTERNAL PRICE

Client provides females for oocyte production and one stud male

 

 

1-5 females as embryo donors

$880

$1,346.40

6-10 females as embryo donors

$1060

$1,621.80