Skip to main content
University of Wisconsin–Madison

Genome Editing & Animal Models

Obtaining Repository Products

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.

The members of the International Knockout Mouse Consortium (IKMC) are working together to mutate all protein-coding genes in the mouse using a combination of gene trapping and gene targeting in C57BL/6 mouse embryonic stem (ES) cells. They have made targeted ES cells, frozen sperm, frozen embryos, and live mice commercially available.

If the mice have already been made, frozen embryos and/or sperm may be available. Before deciding to purchase frozen sperm/embryos from a repository, laboratories must be aware of all the costs involved. Samples will be shipped from the repository to GEAM in a special liquid nitrogen tank. The empty shipping tank must be returned to the repository after delivery of samples. Domestic shipping costs ~ $200, international shipping costs ~ $1000-$1900. Some repositories charge a fee/sample. Live offspring will be recovered from frozen sperm via in vitro fertilization.

Repository Links

RIKEN
KOMP
EUCOMM
EMMA
IKMC

ES cell clones: Please contact Kathy Krentz (kjkrentz@wisc.edu) to get assistance in deciding which targeted clones to purchase. GEAM recommends ordering three mutated ES cell clones; two of which will be injected. Targeted ES cell clones should be karyotyped and mycoplasma tested. Targeted ES cell clones should be sent to:

Genome Editing and Animal Models core
425 Henry Mall
3220 Biotechnology Center
Madison, WI  53706

Frozen embryo/sperm: Please contact Kathy Krentz (kjkrentz@wisc.edu) if you have questions regarding orders. GEAM recommends ordering two vials/straws. Frozen embryo/sperm should be sent to:

Genome Editing and Animal Models core
425 Henry Mall
3220 Biotechnology Center
Madison, WI  53706

 

ES cell expansion $850-$900/clone

ES cell injection into C57BL/6 blastocysts and transfer into pseudopregnant recipients
$1780/microinjection day

Thawing/recovery to live offspring of one vial/straw of frozen mouse embryos $300

Thawing/IVF recovery to live offspring of one vial/straw of frozen mouse sperm $1150-$1460