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University of Wisconsin–Madison

Genome Editing & Animal Models

Sperm Cryopreservation

We are open for business! We are happy to be back. During Phase 1, we will be operating at reduced capacity. We will make every effort to keep turnaround times short as we understand there are many projects that have been waiting. We appreciate your patience and business. The UW Biotechnology Center is following University guidelines regarding COVID-19.
 
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  • Sperm cryopreservation is an inexpensive and efficient means of long-term storage of valuable lines.
  • Sperm cryopreservation works well for single gene mutations on standard genetic backgrounds.
  • The only disadvantage is that live offspring must be recovered through in vitro fertilization (IVF) or intracytoplasmic sperm injections (ICSI).




Mouse sperm cryopreservation has the following steps: 

  1. Contact Kathy Krentz (kjkrentz@wisc.edu) to discuss scheduling
  2. Assessment of animal colony to assure two proven male breeders (per line) would be available to use
  3. Mice are transferred to the Biotechnology center on day of freezing. GEAM will sacrifice males and access sperm motility and concentration
  4. Sperm will be frozen and stored in liquid nitrogen tanks
  5. Recovery of live animals occurs by thawing and performing an IVF then surgically transferring the fertilized embryos into pseudopregant recipients. 

MOUSE SPERM CRYOPRESERVATION (RECOMMEND FREEZING TWO MALES/LINE)

UW PRICE

EXTERNAL PRICE

1 male/day

$275

$425

2 males/day

$475

$730

4 males/day

$800

$1225

8 males/day

$1400

$2175

 

RAT SPERM CRYOPRESERVATION (RECOMMEND FREEZING TWO MALES/LINE)

UW PRICE

EXTERNAL PRICE

1 male/day

$425

$650

2 males/day

$675

$1050

4 males/day

$1,250

$1925