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University of Wisconsin–Madison

Gene Expression Center

Invitrogen Qubit® Fluorometer

The UW-Madison Biotechnology Center is currently open and following the university's COVID-19 Response regarding campus operations.

Room 2302 Independent Access Policy (November 2020)

Please note: Currently, access to the equipment in Biotech Center room 2302 is limited to internal UWBC research groups.

You must notify us of your intent to use the equipment at least 24 hours prior. This allows us to ensure the room is adequately stocked with required supplies in order to maintain appropriate social distancing.

UWBC COVID Room 2302 entry Protocol (11-30-2020)
Please e-mail to request access to the Google calendar for scheduling your usage.

qubit 3.0

The Qubit® 3.0 is a fluorometer capable of measuring low concentrations of several different kinds of analytes, including RNA, DNA and protein. Further information on the Qubit® 3.0 may be found at the supplier's website.

Training is scheduled upon request. The cost of the training is $115/hour plus the cost of reagents. Training typically takes 30 minutes. After being trained to use the fluorometer, a user will have independent access to the instrument.  We use Google Calendar for instrument scheduling.  

Cost $1.75 per sample. Two standards are run every time an assay is performed, and must be counted as samples for billing purposes.  In other words a minimum of three samples will be run, two standards and a user's sample.

Trained users may use their own reagents and consumables.  A $5 access fee will charged for per use.   Data maybe saved to your flash drive*.

The GEC stocks both the dsDNA HS Assay, and the RNA Assay for use with the Qubit.

*Invitrogen tech support does report compatibility issues with some flash drives larger than 2 GB

Effective 4/1/2020

All data and services provided by the University of Wisconsin Biotechnology Center Gene Expression Center are intended for research purposes only. Services are not intended nor certified for diagnostic or clinical use.